Increasing intracellular dNTP levels improves prime editing efficiency

IF 33.1 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Nature biotechnology Pub Date : 2024-09-25 DOI:10.1038/s41587-024-02405-x

Pengpeng Liu, Karthikeyan Ponnienselvan, Thomas Nyalile, Sarah Oikemus, Anya T. Joynt, Sukanya Iyer, Karen Kelly, Dongsheng Guo, Pyae P. Kyawe, Emma Vanderleeden, Sambra D. Redick, Lei Huang, Zexiang Chen, Jeong Min Lee, Celia A. Schiffer, David M. Harlan, Jennifer P. Wang, Charles P. Emerson, Nathan D. Lawson, Jonathan K. Watts, Erik J. Sontheimer, Jeremy Luban, Scot A. Wolfe

引用次数: 0

Abstract

In primary cell types, intracellular deoxynucleotide triphosphate (dNTP) levels are tightly regulated in a cell cycle-dependent manner. We report that prime editing efficiency is increased by mutations that improve the enzymatic properties of Moloney murine leukemia virus reverse transcriptase and treatments that increase intracellular dNTP levels. In combination, these modifications produce substantial increases in precise editing rates.

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增加细胞内 dNTP 水平可提高素材编辑效率

在原代细胞类型中，细胞内脱氧核苷酸三磷酸酯（dNTP）水平以依赖细胞周期的方式受到严格调控。我们报告说，通过突变改善莫罗尼小鼠白血病病毒反转录酶的酶学特性，以及增加细胞内 dNTP 水平的处理方法，可提高质粒编辑效率。将这些修饰结合在一起，可大幅提高精确编辑率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature biotechnology 工程技术-生物工程与应用微生物

CiteScore

63.00

自引率

1.70%

发文量

382

审稿时长

3 months

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