Increasing intracellular dNTP levels improves prime editing efficiency

IF 7.2 2区材料科学 Q2 CHEMISTRY, PHYSICAL

Chemistry of Materials Pub Date : 2024-09-25 DOI:10.1038/s41587-024-02405-x

Pengpeng Liu, Karthikeyan Ponnienselvan, Thomas Nyalile, Sarah Oikemus, Anya T. Joynt, Sukanya Iyer, Karen Kelly, Dongsheng Guo, Pyae P. Kyawe, Emma Vanderleeden, Sambra D. Redick, Lei Huang, Zexiang Chen, Jeong Min Lee, Celia A. Schiffer, David M. Harlan, Jennifer P. Wang, Charles P. Emerson, Nathan D. Lawson, Jonathan K. Watts, Erik J. Sontheimer, Jeremy Luban, Scot A. Wolfe

引用次数: 0

Abstract

In primary cell types, intracellular deoxynucleotide triphosphate (dNTP) levels are tightly regulated in a cell cycle-dependent manner. We report that prime editing efficiency is increased by mutations that improve the enzymatic properties of Moloney murine leukemia virus reverse transcriptase and treatments that increase intracellular dNTP levels. In combination, these modifications produce substantial increases in precise editing rates.

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增加细胞内 dNTP 水平可提高素材编辑效率

在原代细胞类型中，细胞内脱氧核苷酸三磷酸酯（dNTP）水平以依赖细胞周期的方式受到严格调控。我们报告说，通过突变改善莫罗尼小鼠白血病病毒反转录酶的酶学特性，以及增加细胞内 dNTP 水平的处理方法，可提高质粒编辑效率。将这些修饰结合在一起，可大幅提高精确编辑率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemistry of Materials 工程技术-材料科学：综合

CiteScore

14.10

自引率

5.80%

发文量

929

审稿时长

1.5 months

期刊介绍： The journal Chemistry of Materials focuses on publishing original research at the intersection of materials science and chemistry. The studies published in the journal involve chemistry as a prominent component and explore topics such as the design, synthesis, characterization, processing, understanding, and application of functional or potentially functional materials. The journal covers various areas of interest, including inorganic and organic solid-state chemistry, nanomaterials, biomaterials, thin films and polymers, and composite/hybrid materials. The journal particularly seeks papers that highlight the creation or development of innovative materials with novel optical, electrical, magnetic, catalytic, or mechanical properties. It is essential that manuscripts on these topics have a primary focus on the chemistry of materials and represent a significant advancement compared to prior research. Before external reviews are sought, submitted manuscripts undergo a review process by a minimum of two editors to ensure their appropriateness for the journal and the presence of sufficient evidence of a significant advance that will be of broad interest to the materials chemistry community.