Morphological characterization and genetic diversity of mini core collection of Rosa damascena from Morocco

IF 3.5 Q3 Biochemistry, Genetics and Molecular Biology
Nezha Lebkiri , Younes Abbas , Driss Iraqi , Fatima Gaboun , Karim Saghir , Mohamed Fokar , Ismail El hamdi , Khadija Bakhy , Rabha Abdelwahd , Ghizlane Diria
{"title":"Morphological characterization and genetic diversity of mini core collection of Rosa damascena from Morocco","authors":"Nezha Lebkiri ,&nbsp;Younes Abbas ,&nbsp;Driss Iraqi ,&nbsp;Fatima Gaboun ,&nbsp;Karim Saghir ,&nbsp;Mohamed Fokar ,&nbsp;Ismail El hamdi ,&nbsp;Khadija Bakhy ,&nbsp;Rabha Abdelwahd ,&nbsp;Ghizlane Diria","doi":"10.1016/j.jgeb.2024.100423","DOIUrl":null,"url":null,"abstract":"<div><div>Damask rose (<em>Rosa damascena</em> Mill.) is a popular Rosa species cultivated for the industrial production of rose oil worldwide. In the current study, the morphological characteristics and genetic diversity among 12 populations of these species collected from Kelaat M’gouna in Morocco were examined to identify more variable traits and compare their genetic structure. Observations were recorded for a total of 24 morphological traits. The phenotypic variation coefficient (CV) of the studied traits varied from 4.79 % to 42.52 %, confirming the high phenotypic variation between accessions. Cluster analysis grouped accessions into two major clusters based on their morphological resemblance. For molecular investigations, nuclear DNA was amplified using 13 ISSR markers. Analysis of molecular variance (AMOVA) indicated that the highest proportion was within populations (87 %) rather than between them (13 %). Boutaghrar region recorded high values of genetic diversity (He = 0.237), percentage polymorphic loci (PPL = 67 %) and Shannon information index (I = 0.358) Clustering based on Jaccard similarity divided studied the accessions into three distinct clusters. STRUCTURE analysis and principal coordinate analysis (PCoA) were consistent with the genetic relationships derived from cluster analysis. Our study suggests that the wide genetic variation and haplotype observed in Moroccan Damascene roses are valuable for future improvement and conservation of rose programs, particularly for enhancing commercial traits, flower yield, and breeding stability.</div></div>","PeriodicalId":53463,"journal":{"name":"Journal of Genetic Engineering and Biotechnology","volume":"22 4","pages":"Article 100423"},"PeriodicalIF":3.5000,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1687157X24001264/pdfft?md5=ad56b0a7150b88b03593fc0686de25ac&pid=1-s2.0-S1687157X24001264-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Genetic Engineering and Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1687157X24001264","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0

Abstract

Damask rose (Rosa damascena Mill.) is a popular Rosa species cultivated for the industrial production of rose oil worldwide. In the current study, the morphological characteristics and genetic diversity among 12 populations of these species collected from Kelaat M’gouna in Morocco were examined to identify more variable traits and compare their genetic structure. Observations were recorded for a total of 24 morphological traits. The phenotypic variation coefficient (CV) of the studied traits varied from 4.79 % to 42.52 %, confirming the high phenotypic variation between accessions. Cluster analysis grouped accessions into two major clusters based on their morphological resemblance. For molecular investigations, nuclear DNA was amplified using 13 ISSR markers. Analysis of molecular variance (AMOVA) indicated that the highest proportion was within populations (87 %) rather than between them (13 %). Boutaghrar region recorded high values of genetic diversity (He = 0.237), percentage polymorphic loci (PPL = 67 %) and Shannon information index (I = 0.358) Clustering based on Jaccard similarity divided studied the accessions into three distinct clusters. STRUCTURE analysis and principal coordinate analysis (PCoA) were consistent with the genetic relationships derived from cluster analysis. Our study suggests that the wide genetic variation and haplotype observed in Moroccan Damascene roses are valuable for future improvement and conservation of rose programs, particularly for enhancing commercial traits, flower yield, and breeding stability.
摩洛哥大马士革蔷薇迷你核心藏品的形态特征和遗传多样性
大马士革玫瑰(Rosa damascena Mill.)在本研究中,研究人员考察了从摩洛哥 Kelaat M'gouna 采集的 12 个种群的形态特征和遗传多样性,以确定更多的可变性状并比较其遗传结构。共对 24 个形态特征进行了观察记录。所研究性状的表型变异系数(CV)从 4.79 % 到 42.52 % 不等,证实了不同品种之间的表型变异很大。聚类分析根据不同品种的形态相似性将其分为两大类。为了进行分子研究,使用 13 个 ISSR 标记扩增了核 DNA。分子方差分析(AMOVA)表明,种群内部的差异比例最高(87%),而种群之间的差异比例最低(13%)。布塔格拉尔地区的遗传多样性(He = 0.237)、多态性位点百分比(PPL = 67 %)和香农信息指数(I = 0.358)都很高。结构分析和主坐标分析(PCoA)与聚类分析得出的遗传关系一致。我们的研究表明,在摩洛哥大马士革玫瑰中观察到的广泛遗传变异和单倍型对未来玫瑰改良和保护项目很有价值,特别是在提高商业性状、花朵产量和育种稳定性方面。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Genetic Engineering and Biotechnology
Journal of Genetic Engineering and Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
5.70
自引率
5.70%
发文量
159
审稿时长
16 weeks
期刊介绍: Journal of genetic engineering and biotechnology is devoted to rapid publication of full-length research papers that leads to significant contribution in advancing knowledge in genetic engineering and biotechnology and provide novel perspectives in this research area. JGEB includes all major themes related to genetic engineering and recombinant DNA. The area of interest of JGEB includes but not restricted to: •Plant genetics •Animal genetics •Bacterial enzymes •Agricultural Biotechnology, •Biochemistry, •Biophysics, •Bioinformatics, •Environmental Biotechnology, •Industrial Biotechnology, •Microbial biotechnology, •Medical Biotechnology, •Bioenergy, Biosafety, •Biosecurity, •Bioethics, •GMOS, •Genomic, •Proteomic JGEB accepts
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信