Modulatory Effects of Isolated Lactobacillus paracasei from Malaysian Water Kefir Grains on the Intestinal Barrier and Gut Microbiota in Diabetic Mice.

IF 4.4 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Noorshafadzilah Talib, Nurul Elyani Mohamad, Chai Ling Ho, Mas Jaffri Masarudin, Noorjahan Banu Alitheen
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Abstract

Type 2 diabetes (T2DM) is one of the four major types of non-communicable diseases that have become a global health concern. Water kefir is a product of a brown sugar solution fermented with kefir grains which comprises around 30 microbial species in its grains. Water kefir possesses a wide range of health benefits, including anti-hyperlipidemic effects, and reduces hypertension and blood glucose levels in animal models. Reportedly, consuming water kefir containing probiotics may enhance the intestinal barrier and positively influence the composition of the intestinal microflora. The present study aimed to evaluate the regulatory effects of Lactobacillus paracasei isolated from Malaysian water kefir grains (MWKG) on the alterations of intestinal barrier and gut microbiota in diabetic mice via histopathological analysis of the distal colon and 16S rRNA gene sequencing on fecal microbiome. Results indicated that the administration of isolated Lactobacillus paracasei from MWKG to diabetic mice ameliorated the dominant probiotic phyla in the gut microbiota. Results showed that lower dose (LD) and high dose (HD) treatments of the isolated Lactobacillus paracasei could significantly reduce inflammatory cell infiltration in the distal colon of diabetic mice. The treatments revealed a significant decrease in the relative abundance of Firmicutes in the gut, 0.27 ± 0.06% for LD and 0.34 ± 0.04% for HD, compared to untreated (UN) diabetic mice, 0.40 ± 0.02%. These results suggest that L. paracasei isolated from MWKG could serve as a potential dietary supplement against intestinal inflammation and modify gut microbiota composition in patients with T2DM.

马来西亚克菲尔水粒中分离出的副卡西氏乳杆菌对糖尿病小鼠肠道屏障和肠道微生物群的调节作用
2 型糖尿病(T2DM)是四大非传染性疾病之一,已成为全球关注的健康问题。水酸乳是一种用红糖溶液与酸乳谷物发酵而成的产品,其谷物中含有约 30 种微生物。开菲尔水具有多种健康益处,包括抗高血脂作用、降低动物模型的高血压和血糖水平。据报道,饮用含有益生菌的水酸乳可增强肠道屏障,并对肠道微生物区系的组成产生积极影响。本研究旨在通过对远端结肠的组织病理学分析和对粪便微生物组的 16S rRNA 基因测序,评估从马来西亚水凯菲尔谷物(MWKG)中分离出的副干酪乳杆菌对糖尿病小鼠肠道屏障和肠道微生物区系改变的调节作用。结果表明,糖尿病小鼠服用从 MWKG 中分离出的副干酪乳杆菌后,肠道微生物群中的优势益生菌群得到改善。研究结果表明,低剂量(LD)和高剂量(HD)分离的副干酪乳杆菌能显著减少糖尿病小鼠远端结肠的炎症细胞浸润。与未经处理(UN)的糖尿病小鼠(0.40 ± 0.02%)相比,处理显示肠道中固有菌的相对丰度明显下降,低剂量为 0.27 ± 0.06%,高剂量为 0.34 ± 0.04%。这些结果表明,从MWKG中分离出的帕拉卡酶L.可作为一种潜在的膳食补充剂,对抗肠道炎症,并改变T2DM患者的肠道微生物群组成。
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来源期刊
Probiotics and Antimicrobial Proteins
Probiotics and Antimicrobial Proteins BIOTECHNOLOGY & APPLIED MICROBIOLOGYMICROB-MICROBIOLOGY
CiteScore
11.30
自引率
6.10%
发文量
140
期刊介绍: Probiotics and Antimicrobial Proteins publishes reviews, original articles, letters and short notes and technical/methodological communications aimed at advancing fundamental knowledge and exploration of the applications of probiotics, natural antimicrobial proteins and their derivatives in biomedical, agricultural, veterinary, food, and cosmetic products. The Journal welcomes fundamental research articles and reports on applications of these microorganisms and substances, and encourages structural studies and studies that correlate the structure and functional properties of antimicrobial proteins.
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