Osteogenic effect of poly(lactic-co-glycolic acid) microcapsules with different molecular weights encapsulating bone morphogenetic protein 2.

Lihong Yuan, Chen Chen, Yudi Ma, Ruizhen Liang
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Abstract

Objectives: This study aimed to explore the effects of bone morphogenetic protein 2 (BMP-2) encapsula-ted in poly(lactic-co-glycolic acid) (PLGA) microcapsules with different molecular weights on the osteogenic ability of osteoblasts.

Methods: PLGA microcapsules with different molecular weights (12 000, 30 000) encapsulating BMP-2, were prepared using a dual-channel microinjection pump. The morphology and structure of the microcapsules were characterized by optical microscopy and scanning electron microscopy. The sustained-release performance of the microcapsules was characterized by phosphate buffered saline immersion method. The cell compatibility of the microcapsules was detected by the Calcein-AM/PI staining and CCK-8 method. The chemotactic effect of BMP-2-encapsulated microcapsules on MC3T3-E1 cells after 48 h of treatment was detected by the Transwell assay. The alkaline phosphatase activity assay and Alizarin Red S staining were used to characterize the effect of microcapsules on the osteogenic ability of MC3T3-E1 cells.

Results: Both types of microcapsules with different molecular weights exhibited smooth surfaces, as well as uniform and good cell compatibility. The chemotactic effect of the 12 000 microcapsules was outstanding. The 30 000 microcapsules had a longer sustained-release time, and the initial burst release was reduced by approximately 25% compared with the 12 000 microcapsules. In addition, 30 000 microcapsules performed better in long-term osteogenesis induction than 12 000 microcapsules.

Conclusions: In this study, the release of BMP-2 is regulated by adjusting the molecular weight of PLGA, and the results indicate that 30 000 microcapsules can better induce the long-term osteogenic ability of MC3T3-E1 cells.

不同分子量的包裹骨形态发生蛋白 2 的聚(乳酸-共-乙醇酸)微胶囊的成骨效应。
研究目的本研究旨在探讨不同分子量的聚乳酸-聚乙二醇酸(PLGA)微胶囊包裹骨形态发生蛋白 2(BMP-2)对成骨细胞成骨能力的影响:方法:使用双通道微量注射泵制备了不同分子量(12 000、30 000)的包裹 BMP-2 的 PLGA 微胶囊。光学显微镜和扫描电子显微镜对微胶囊的形态和结构进行了表征。采用磷酸盐缓冲盐水浸泡法对微胶囊的缓释性能进行了表征。钙黄绿素-AM/PI染色法和CCK-8法检测了微胶囊的细胞相容性。Transwell 试验检测了处理 48 小时后 BMP-2 包囊微胶囊对 MC3T3-E1 细胞的趋化作用。碱性磷酸酶活性测定和茜素红 S 染色法表征了微胶囊对 MC3T3-E1 细胞成骨能力的影响:结果:两种不同分子量的微胶囊都表现出表面光滑、均匀和良好的细胞相容性。12 000 微胶囊的趋化效果显著。与 12 000 微胶囊相比,30 000 微胶囊的持续释放时间更长,初始爆发释放量减少了约 25%。此外,与 12 000 微胶囊相比,30 000 微胶囊的长期成骨诱导效果更好:本研究通过调节 PLGA 的分子量来调节 BMP-2 的释放,结果表明 30 000 微胶囊能更好地诱导 MC3T3-E1 细胞的长期成骨能力。
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