Evaluating Mechanisms of Soil Microbiome Suppression of Striga Infection in Sorghum.

IF 1 Q3 BIOLOGY
Tamera Taylor, Jiregna Daksa, Mahdere Z Shimels, Desalegn W Etalo, Benjamin Thiombiano, Aimee Walmsey, Alexander J Chen, Harro J Bouwmeester, Jos M Raaijmakers, Siobhan M Brady, Dorota Kawa
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Abstract

The root parasitic weed Striga hermonthica has a devastating effect on sorghum and other cereal crops in Sub-Saharan Africa. Available Striga management strategies are rarely sufficient or not widely accessible or affordable. Identification of soil- or plant-associated microorganisms that interfere in the Striga infection cycle holds potential for development of complementary biological control measures. Such inoculants should be preferably based on microbes native to the regions of their application. We developed a method to assess microbiome-based soil suppressiveness to Striga with a minimal amount of field-collected soil. We previously used this method to identify the mechanisms of microbe-mediated suppression of Striga infection and to test individual microbial strains. Here, we present protocols to assess the functional potential of the soil microbiome and individual bacterial taxa that adversely affect Striga parasitism in sorghum via three major known suppression mechanisms. These methods can be further extended to other Striga hosts and other root parasitic weeds. Key features • This protocol provides a detailed description of the methods used in Kawa et al. [1]. • This protocol is optimized to assess soil suppressiveness to Striga infection by using natural field-collected soil and the same soil sterilized by gamma-radiation. • This protocol is optimized to test bacterial (and not fungal) isolates. • This protocol can be easily extended to other host-parasite-microbiome systems.

评估土壤微生物组抑制高粱Striga感染的机制
根部寄生杂草 Striga hermonthica 对撒哈拉以南非洲地区的高粱和其他谷类作物具有毁灭性影响。现有的 Striga 防治策略很少足够有效,或者不能广泛使用,或者负担不起。鉴定与土壤或植物相关的、能干扰 Striga 感染循环的微生物,有可能开发出补充性生物防治措施。此类接种剂最好以应用地区的本地微生物为基础。我们开发了一种方法,用最少的田间采集土壤来评估基于微生物的土壤对 Striga 的抑制能力。此前,我们曾用这种方法确定了微生物介导的抑制 Striga 感染的机制,并对单个微生物菌株进行了测试。在此,我们提出了评估土壤微生物组和单个细菌类群功能潜力的方案,这些细菌类群通过三种已知的主要抑制机制对高粱中的斯里加寄生虫产生不利影响。这些方法可进一步扩展到其他 Striga 宿主和其他根部寄生杂草。主要特点 - 本方案详细介绍了 Kawa 等人[1]所使用的方法。- 本方案经过优化,可使用田间采集的天然土壤和经过伽马射线消毒的相同土壤来评估土壤对 Striga 感染的抑制能力。- 本方案优化用于测试细菌(而非真菌)分离物。- 该方案可轻松扩展到其他宿主-寄生虫-微生物组系统。
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1.50
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