Stereological study of cerebellar morphology in feline fetuses: Insights from the final gestational stage

IF 2 3区 医学 Q2 ANATOMY & MORPHOLOGY
Vinícius Pedro Silva de Oliveira , Tais Harumi de Castro Sasahara , Gabriela Salim de Castro , Lara Carolina Mario , Bruno Cesar Schimming , José Roberto Kfoury Júnior , Silvio Pires Gomes
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Abstract

This study aimed to conduct a morphoquantitative and stereological evaluation, analyzing the cerebellum of domestic cat fetuses in the latter third of the gestational period. Fetal samples were obtained from a neutering campaign conducted in the municipality of Guarulhos, São Paulo, Brazil. The procedures and protocols used in this work adhere to the guidelines established by the ethics committee of the School of Veterinary Medicine and Animal Science at the University of São Paulo (FMVZ/USP), under the number CEUA 1935251121. The five selected fetuses were fixed in 4 % formaldehyde, and their gestational age was determined by Crown Rump (CR) measurements, followed by an assessment of external characteristics. The cerebella were subjected to the evaluation of morphometric parameters and histological processing using stereology techniques. The obtained means for the cerebellar parameters were as follows: length: 1.0-centimeter, width: 0.54 centimeters, thickness: 0.44 centimeters, and weight: 0.84 g. Using stereology, the following parameters were determined: cerebellar volume, averaging 0.847 cm³; volume density of the cortex: 0.496 or 49 % (molecular layer), 0.0314 or 3.14 % (Purkinje cell layer), 0.232 or 23 % (granular layer), and 0.234 or 23 % (medullary white center). Consequently, the average total volume of the cerebellar cortex is 0.419 cm³ for the molecular layer, 0.026 cm³ for the Purkinje cell layer, 0.196 cm³ for the granular layer, and 0.196 cm³ for the medullary white center. The findings presented here have contributed to an in-depth discussion of the neuro-motor development and cerebellum of domestic cats.
猫科胎儿小脑形态的立体学研究:妊娠末期的启示
本研究旨在对妊娠期后三分之一的家猫胎儿的小脑进行形态定量和立体学评估分析。胎儿样本来自巴西圣保罗瓜鲁柳斯市的一次绝育手术。这项工作中使用的程序和方案符合圣保罗大学兽医和动物科学学院(FMVZ/USP)伦理委员会制定的指导方针,编号为 CEUA 1935251121。所选的五个胎儿被固定在4%的甲醛中,通过测量冠臀围(CR)确定胎龄,然后评估外部特征。对小脑进行形态计量参数评估,并利用立体学技术进行组织学处理。小脑参数的平均值如下:长:1.0 厘米,宽:0.54 厘米,厚:0.44 厘米,重:0.84 克。使用立体学方法确定了以下参数:小脑体积,平均为 0.847 立方厘米;皮层体积密度:0.496 或 49%(分子层),0.0314 或 3.14%(浦肯野细胞层),0.232 或 23%(颗粒层),以及 0.234 或 23%(髓质白中心)。因此,小脑皮质的平均总体积为:分子层 0.419 立方厘米,浦肯野细胞层 0.026 立方厘米,颗粒层 0.196 立方厘米,髓白中心 0.196 立方厘米。本文的研究结果有助于深入探讨家猫的神经运动发育和小脑。
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来源期刊
Annals of Anatomy-Anatomischer Anzeiger
Annals of Anatomy-Anatomischer Anzeiger 医学-解剖学与形态学
CiteScore
4.40
自引率
22.70%
发文量
137
审稿时长
33 days
期刊介绍: Annals of Anatomy publish peer reviewed original articles as well as brief review articles. The journal is open to original papers covering a link between anatomy and areas such as •molecular biology, •cell biology •reproductive biology •immunobiology •developmental biology, neurobiology •embryology as well as •neuroanatomy •neuroimmunology •clinical anatomy •comparative anatomy •modern imaging techniques •evolution, and especially also •aging
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