Hydrogen sulfide antagonizes cytokinin to change root system architecture through persulfidation of CKX2 in Arabidopsis

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2024-09-15 DOI:10.1111/nph.20122

Xiuyu Wang, Cuixia Liu, Tian Li, Fangyu Zhou, Haotian Sun, Fali Li, Ying Ma, Honglei Jia, Xiaoyue Zhang, Wei Shi, Chunmei Gong, Jisheng Li

{"title":"Hydrogen sulfide antagonizes cytokinin to change root system architecture through persulfidation of CKX2 in Arabidopsis","authors":"Xiuyu Wang, Cuixia Liu, Tian Li, Fangyu Zhou, Haotian Sun, Fali Li, Ying Ma, Honglei Jia, Xiaoyue Zhang, Wei Shi, Chunmei Gong, Jisheng Li","doi":"10.1111/nph.20122","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n \n <ul>\n \n \n <li>Hydrogen sulfide (H2S) is an endogenous gaseous signaling molecule, which has been shown to play an important role in plant growth and development by coupling with various phytohormones. However, the relationship between H2S and cytokinin (CTK) and the mechanisms by which H2S and CTK affect root growth remain poorly understood.</li>\n \n \n <li>Endogenous CTK was analyzed by UHPLC-ESI-MS/MS. Persulfidation of cytokinin oxidase/dehydrogenases (CKXs) was analyzed by mass spectrometry (MS). ckx2/CKX2wild-type (WT), OE CKX2 and ckx2/CKX2Cys(C)62alanine(A) transgenic lines were isolated with the ckx2 background.</li>\n \n \n <li>H2S is linked to CTK content by CKX2, which regulates root system architecture (RSA). Persulfidation at cysteine (Cys)62 residue of CKX2 enhances CKX2 activity, resulting in reduced CTK content. We utilized 35S-LCD/oasa1 transgenic lines to investigate the effect of endogenous H2S on RSA, indicating that H2S reduces the gravitropic set-point angle (GSA), shortens root hairs, and increases the number of lateral roots (LRs). The persulfidation of CKX2Cys62 changes the elongation of cells on the upper and lower flanks of LR elongation zone, confirming that Cys62 of CKX2 is the specificity target of H2S to regulate RSA in vivo.</li>\n \n \n <li>In conclusion, this study demonstrated that H2S negatively regulates CTK content and affects RSA by persulfidation of CKX2Cys62 in Arabidopsis thaliana.</li>\n </ul>\n \n </div>","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":null,"pages":null},"PeriodicalIF":8.3000,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Phytologist","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/nph.20122","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Hydrogen sulfide (H₂S) is an endogenous gaseous signaling molecule, which has been shown to play an important role in plant growth and development by coupling with various phytohormones. However, the relationship between H₂S and cytokinin (CTK) and the mechanisms by which H₂S and CTK affect root growth remain poorly understood.
Endogenous CTK was analyzed by UHPLC-ESI-MS/MS. Persulfidation of cytokinin oxidase/dehydrogenases (CKXs) was analyzed by mass spectrometry (MS). ckx2/CKX2^{wild-type (WT)}, OE CKX2 and ckx2/CKX2^{Cys(C)62alanine(A)} transgenic lines were isolated with the ckx2 background.
H₂S is linked to CTK content by CKX2, which regulates root system architecture (RSA). Persulfidation at cysteine (Cys)62 residue of CKX2 enhances CKX2 activity, resulting in reduced CTK content. We utilized 35S-LCD/oasa1 transgenic lines to investigate the effect of endogenous H₂S on RSA, indicating that H₂S reduces the gravitropic set-point angle (GSA), shortens root hairs, and increases the number of lateral roots (LRs). The persulfidation of CKX2^Cys62 changes the elongation of cells on the upper and lower flanks of LR elongation zone, confirming that Cys62 of CKX2 is the specificity target of H₂S to regulate RSA in vivo.
In conclusion, this study demonstrated that H₂S negatively regulates CTK content and affects RSA by persulfidation of CKX2^Cys62 in Arabidopsis thaliana.

查看原文本刊更多论文

硫化氢通过拟南芥中 CKX2 的过硫化作用拮抗细胞分裂素，从而改变根系结构。

硫化氢（H2S）是一种内源性气态信号分子，已被证明可通过与各种植物激素的耦合作用在植物生长和发育过程中发挥重要作用。然而，人们对 H2S 和细胞分裂素（CTK）之间的关系以及 H2S 和 CTK 影响根系生长的机制仍然知之甚少。采用超高效液相色谱-电喷雾离子交换-质谱（UHPLC-ESI-MS/MS）分析了内源 CTK。以ckx2为背景分离出了ckx2/CKX2野生型（WT）、OE CKX2和ckx2/CKX2Cys(C)62丙氨酸(A)转基因品系。CKX2 将 H2S 与 CTK 含量联系起来，从而调节根系结构（RSA）。CKX2半胱氨酸（Cys）62残基的过硫化增强了CKX2的活性，导致CTK含量降低。我们利用 35S-LCD/oasa1 转基因品系研究了内源 H2S 对 RSA 的影响，结果表明 H2S 会降低重力定点角（GSA）、缩短根毛并增加侧根（LR）的数量。CKX2Cys62 的过硫化作用改变了侧根伸长区上下两翼细胞的伸长，证实了 CKX2 的 Cys62 是 H2S 在体内调控侧根伸长区的特异性靶标。总之，本研究证明了 H2S 通过过硫化 CKX2Cys62 负向调节拟南芥中 CTK 的含量并影响 RSA。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.