Conditions for improved accuracy of noninvasive preimplantation genetic testing for aneuploidy: Focusing on the zona pellucida and early blastocysts.

IF 2.7 3区医学 Q2 OBSTETRICS & GYNECOLOGY

Reproductive Medicine and Biology Pub Date : 2024-09-10 eCollection Date: 2024-01-01 DOI:10.1002/rmb2.12604

Hiroki Takeuchi, Midori Morishita, Midori Uemura, Tadashi Maezawa, Takashi Shibahara, Erina Takayama, Mikiko Nishioka, Eiji Kondo, Hiroyuki Minoura, Tomoaki Ikeda

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Abstract

Purpose: Recently, noninvasive preimplantation genetic testing for aneuploidy (niPGT-A) using cell-free deoxyribonucleic acid has been developed; however, there are few reports on this and the results are inconsistent. This study was conducted to optimize the cultural environment.

Methods: We used 35 blastocysts that had been discarded after in-vitro fertilization. The concordance rate of karyotype analysis results between whole embryos (WEs), spent culture mediums (SCMs), and trophectoderms after 8, 16, and 24 h of culture was examined. Next, zona pellucida (ZP)-free blastocysts and then early blastocysts were cultured for 24 h each.

Results: Regarding the optimal culture times, the concordance rate between WEs and SCMs was 20%, 60%, and 100% at 8, 16, and 24 h, respectively. Significant differences were found between 8 and 24 h. The concordance rate with ZP cultures was 40.0%, and no significant differences were found. The concordance rate of early blastocysts thawed and cultured for 24 h was 40.0%, which was significantly lower than that of day 5 blastocysts.

Conclusions: The optimal culture times for niPGT-A were 24 h, and the concordance rate with free ZP was higher. The concordance rate for early blastocysts was low, suggesting that optimization of the conditions may be necessary.

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提高非整倍体植入前无创性基因检测准确性的条件：关注透明带和早期囊胚。

目的：最近，利用无细胞脱氧核糖核酸进行非整倍体植入前基因检测（niPGT-A）得到了发展；然而，这方面的报道很少，结果也不一致。本研究旨在优化培养环境：我们使用了 35 个体外受精后废弃的囊胚。方法：我们使用了 35 个体外受精后丢弃的囊胚，对培养 8、16 和 24 小时后的全胚（WE）、废培养基（SCM）和滋养层胚胎的核型分析结果的一致性进行了检测。接着，对无透明带（ZP）囊胚和早期囊胚各培养 24 小时：结果：关于最佳培养时间，在 8、16 和 24 小时内，WEs 和 SCMs 的一致率分别为 20%、60% 和 100%。与 ZP 培养物的吻合率为 40.0%，未发现显著差异。解冻并培养 24 小时的早期囊胚的吻合率为 40.0%，明显低于第 5 天囊胚的吻合率：结论：niPGT-A 的最佳培养时间为 24 小时，与游离 ZP 的吻合率较高。结论：niPGT-A 的最佳培养时间为 24 小时，与游离 ZP 的吻合率较高，而早期囊胚的吻合率较低，这表明有必要优化培养条件。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproductive Medicine and Biology Multiple-

CiteScore

5.70

自引率

5.90%

发文量

审稿时长

20 weeks

期刊介绍： Reproductive Medicine and Biology (RMB) is the official English journal of the Japan Society for Reproductive Medicine, the Japan Society of Fertilization and Implantation, the Japan Society of Andrology, and publishes original research articles that report new findings or concepts in all aspects of reproductive phenomena in all kinds of mammals. Papers in any of the following fields will be considered: andrology, endocrinology, oncology, immunology, genetics, function of gonads and genital tracts, erectile dysfunction, gametogenesis, function of accessory sex organs, fertilization, embryogenesis, embryo manipulation, pregnancy, implantation, ontogenesis, infectious disease, contraception, etc.