Expression of RAG and secondary gene rearrangement of BCR in mature peripheral B lymphocytes in Takayasu arteritis.

IF 4.9 3区 医学 Q2 IMMUNOLOGY
Immunology Pub Date : 2025-01-01 Epub Date: 2024-09-21 DOI:10.1111/imm.13865
Zhenni Wang, Zongxu Jing, Xiaoyun Luo
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引用次数: 0

Abstract

To evaluate the expression of recombinant activating gene (RAG) and B cell receptor (BCR) gene rearrangements in mature peripheral B lymphocytes in Takayasu arteritis (TA) to explore the possible mechanism of humoral immune response in TA. Ten patients with TA and 10 age- and sex-matched healthy volunteers (control group) from Beijing Shijitan Hospital, Capital Medical University and Peking Union Medical College Hospital, between 2022 and 2023, were included in this study. The mRNA of the RAG was measured using real-time quantitative PCR (RT-PCR). Western blotting was used to detect RAG protein expression levels. NGS technology was used to detect BCR gene rearrangement. The mRNA expression level of RAG1 and RAG2 in peripheral mature B lymphocytes in patients with TA was significantly higher than in the control group (RAG1 5.56 ± 1.71 vs. 1.94 ± 0.86, p < 0.05; RAG2 5.26 ± 1.59 vs. 1.65 ± 0.64, p < 0.05), respectively. The protein expression level of the RAG1 and the RAG2 in peripheral mature B lymphocytes in patients with TA was significantly higher than in the healthy control group (RAG1 4.33 ± 1.58 vs. 1.52 ± 0.59, p < 0.001; RAG2 4.67 ± 1.88 vs. 1.59 ± 0.56, p < 0.001). The number of peripheral B lymphocyte BCR clonotypes in the group of patients with TA was significantly higher than in the normal control group (1574 ± 317.7 vs. 801.3 ± 202.1, p < 0.05). The abundance of IGHV clones in patients with TA was higher than in the normal control group (31.185% vs. 13.449%), which was positively correlated with the expression levels of RAG1 and RAG2 (correlation coefficient r = 1.00, p < 0.001), respectively. High expression of the RAG gene coexists with secondary BCR gene rearrangement in mature peripheral B lymphocytes in patients with TA, providing important clues regarding the potential humoral response in TA; however, further studies with larger samples are needed.

高安动脉炎患者成熟外周 B 淋巴细胞中 RAG 的表达和 BCR 的次级基因重排。
评估重组激活基因(RAG)和B细胞受体(BCR)基因重排在高安动脉炎(TA)成熟外周B淋巴细胞中的表达,探讨TA体液免疫反应的可能机制。本研究纳入了2022年至2023年间来自首都医科大学附属北京世纪坛医院和北京协和医院的10名TA患者和10名年龄和性别匹配的健康志愿者(对照组)。采用实时定量 PCR(RT-PCR)技术检测 RAG 的 mRNA。采用 Western 印迹法检测 RAG 蛋白表达水平。NGS技术用于检测BCR基因重排。TA患者外周成熟B淋巴细胞中RAG1和RAG2的mRNA表达水平明显高于对照组(RAG1 5.56 ± 1.71 vs. 1.94 ± 0.86, p
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来源期刊
Immunology
Immunology 医学-免疫学
CiteScore
11.90
自引率
1.60%
发文量
175
审稿时长
4-8 weeks
期刊介绍: Immunology is one of the longest-established immunology journals and is recognised as one of the leading journals in its field. We have global representation in authors, editors and reviewers. Immunology publishes papers describing original findings in all areas of cellular and molecular immunology. High-quality original articles describing mechanistic insights into fundamental aspects of the immune system are welcome. Topics of interest to the journal include: immune cell development, cancer immunology, systems immunology/omics and informatics, inflammation, immunometabolism, immunology of infection, microbiota and immunity, mucosal immunology, and neuroimmunology. The journal also publishes commissioned review articles on subjects of topical interest to immunologists, and commissions in-depth review series: themed sets of review articles which take a 360° view of select topics at the heart of immunological research.
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