Evaluation of the effect of taurine on the matrix metalloproteinase-9 and the expression changes of miRNA-21 and miRNA-146a in SH-SY5Y cell line.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-09-16 DOI:10.1515/hmbci-2024-0005

Abduladheem Al-Attabi, Bilal Abdulmajeed Mukhlif, Karrar R Al-Shami, Muna S Merza, Sami Awad Alkubaisy, Mohanad Ali Abdulhadi

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引用次数: 0

Abstract

Objectives: Alzheimer's disease (AD), a brain disorder, is the leading cause of dementia among older adults. Taurine, an amino acid abundantly present in the brain, and shows potential neuroprotective properties. Therefore, we investigated the effects of taurine on Matrix Metalloproteinase-9 (MMP-9) levels and the expression changes of miRNA-21 and miRNA-146a in the SH-SY5Y cell line.

Methods: Taurine's impact on the SH-SY5Y cell line was evaluated via the 2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. MMP-9 levels were measured using an enzyme-linked immunosorbent assay (ELISA) kit, while the expression of miRNA-21 and miRNA-146a genes was assessed through Real-Time PCR analysis.

Results: The MTT assay revealed no toxic effects on SH-SY5Y cells with increasing concentrations of taurine. Analysis of gene expression indicated a rise in miRNA-21 expression and a decline in miRNA-146 expression with increasing taurine concentration, with the most notable change observed at 1 mg/mL taurine (p<0.001). ELISA results demonstrated a significant increase in MMP-9 levels in the SH-SY5Y cell line treated with 1 mg/mL taurine compared to the untreated group (p<0.001).

Conclusions: Our study revealed that taurine can alter the expression of miRNA-146a and miRNA-21. In conclusion, taurine therapy presents promising therapeutic avenues for treating AD or mitigating severe symptoms. Nonetheless, further research is necessary to comprehensively grasp the precise mechanisms at play.

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评估牛磺酸对基质金属蛋白酶-9的影响以及miRNA-21和miRNA-146a在SH-SY5Y细胞系中的表达变化。

目标：阿尔茨海默病（AD）是一种脑部疾病，是导致老年人痴呆的主要原因。牛磺酸是一种在大脑中含量丰富的氨基酸，具有潜在的神经保护特性。因此，我们研究了牛磺酸对基质金属蛋白酶-9（MMP-9）水平的影响，以及 miRNA-21 和 miRNA-146a 在 SH-SY5Y 细胞系中的表达变化：方法：牛磺酸对SH-SY5Y细胞系的影响通过2,5-二苯基-2H-溴化四氮唑（MTT）试验进行评估。使用酶联免疫吸附试验（ELISA）试剂盒测定了 MMP-9 的水平，并通过实时 PCR 分析评估了 miRNA-21 和 miRNA-146a 基因的表达：结果：MTT 试验表明，随着牛磺酸浓度的增加，SH-SY5Y 细胞不会受到毒性影响。基因表达分析表明，随着牛磺酸浓度的增加，miRNA-21 的表达上升，miRNA-146 的表达下降，在 1 mg/mL 牛磺酸浓度下观察到的变化最为显著（p结论：我们的研究表明，牛磺酸能改变 miRNA-146a 和 miRNA-21 的表达。总之，牛磺酸疗法为治疗注意力缺失症或减轻严重症状提供了有希望的治疗途径。然而，要全面掌握其确切的作用机制，还需要进一步的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.