Salivary Calprotectin Levels and Bone Mineral Density in Post-Menopausal Women With and Without Chronic Periodontitis - An Evaluative Study.

Q3 Dentistry
Indian Journal of Dental Research Pub Date : 2024-04-01 Epub Date: 2024-09-14 DOI:10.4103/ijdr.ijdr_522_21
J Pavithra, Amitha Ramesh, Biju Thomas, N Suchetha Kumari, K P Sharmila
{"title":"Salivary Calprotectin Levels and Bone Mineral Density in Post-Menopausal Women With and Without Chronic Periodontitis - An Evaluative Study.","authors":"J Pavithra, Amitha Ramesh, Biju Thomas, N Suchetha Kumari, K P Sharmila","doi":"10.4103/ijdr.ijdr_522_21","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Menopause is a normal developmental stage in a woman's life marking the permanent cessation of menstruation. Periodontitis and osteoporosis are noteworthy health concerns in post-menopausal women. Calprotectin is an inflammatory biomarker for precise diagnosis of periodontal disease.</p><p><strong>Materials and methods: </strong>This study was performed to evaluate the variations in salivary calprotectin levels and bone mineral density in post-menopausal women with and without chronic periodontitis. The study included 24 post-menopausal women aged 45-57 years and was divided into group 1 (12 post-menopausal women without chronic periodontitis) and group 2 (12 post-menopausal women with chronic periodontitis). Unstimulated salivary samples, panoramic radiographs and clinical examination were performed.</p><p><strong>Results: </strong>The collected samples were combined with CALPRO antibody with horseradish peroxide (HRP) that results in the formation of antigen-antibody complex. Tetramethylbenzidine (TMB) solution was added that turned blue. Colour change was measured at 450nm. A human ELISA kit was used. Data collected were sent for statistical analysis.</p><p><strong>Discussion: </strong>There was a statistically significant difference in salivary calprotectin levels between groups 1 and 2 ( P = 0.016) and no statistical difference in mean bone mineral density levels between the two groups ( P = 0.235). A positive correlation was seen between salivary calprotectin levels and bone mineral density in group 2 ( P = 0.013).</p>","PeriodicalId":13311,"journal":{"name":"Indian Journal of Dental Research","volume":" ","pages":"140-144"},"PeriodicalIF":0.0000,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Dental Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/ijdr.ijdr_522_21","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/14 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"Dentistry","Score":null,"Total":0}
引用次数: 0

Abstract

Introduction: Menopause is a normal developmental stage in a woman's life marking the permanent cessation of menstruation. Periodontitis and osteoporosis are noteworthy health concerns in post-menopausal women. Calprotectin is an inflammatory biomarker for precise diagnosis of periodontal disease.

Materials and methods: This study was performed to evaluate the variations in salivary calprotectin levels and bone mineral density in post-menopausal women with and without chronic periodontitis. The study included 24 post-menopausal women aged 45-57 years and was divided into group 1 (12 post-menopausal women without chronic periodontitis) and group 2 (12 post-menopausal women with chronic periodontitis). Unstimulated salivary samples, panoramic radiographs and clinical examination were performed.

Results: The collected samples were combined with CALPRO antibody with horseradish peroxide (HRP) that results in the formation of antigen-antibody complex. Tetramethylbenzidine (TMB) solution was added that turned blue. Colour change was measured at 450nm. A human ELISA kit was used. Data collected were sent for statistical analysis.

Discussion: There was a statistically significant difference in salivary calprotectin levels between groups 1 and 2 ( P = 0.016) and no statistical difference in mean bone mineral density levels between the two groups ( P = 0.235). A positive correlation was seen between salivary calprotectin levels and bone mineral density in group 2 ( P = 0.013).

患有和未患有慢性牙周炎的绝经后妇女的唾液钙粘蛋白水平和骨矿物质密度--一项评估性研究。
简介更年期是女性一生中正常的发育阶段,标志着月经的永久停止。牙周炎和骨质疏松症是绝经后妇女值得关注的健康问题。钙粘蛋白是一种炎症生物标志物,可用于牙周疾病的精确诊断:本研究旨在评估患有和未患有慢性牙周炎的绝经后妇女唾液钙蛋白水平和骨矿物质密度的变化。研究包括 24 名 45-57 岁的绝经后妇女,分为第一组(12 名绝经后妇女,无慢性牙周炎)和第二组(12 名绝经后妇女,有慢性牙周炎)。研究人员采集了未受刺激的唾液样本,并进行了全景放射摄影和临床检查:采集的样本与带有过氧化辣根(HRP)的 CALPRO 抗体结合,形成抗原抗体复合物。加入四甲基联苯胺(TMB)溶液,使其变成蓝色。在 450 纳米波长下测量颜色变化。使用的是人类 ELISA 试剂盒。收集的数据被送去进行统计分析:第 1 组和第 2 组的唾液钙蛋白水平差异有统计学意义(P = 0.016),而两组的平均骨矿密度水平无统计学差异(P = 0.235)。第 2 组的唾液钙蛋白水平与骨矿物质密度呈正相关(P = 0.013)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Indian Journal of Dental Research
Indian Journal of Dental Research Dentistry-Dentistry (all)
CiteScore
1.80
自引率
0.00%
发文量
80
审稿时长
38 weeks
期刊介绍: Indian Journal of Dental Research (IJDR) is the official publication of the Indian Society for Dental Research (ISDR), India section of the International Association for Dental Research (IADR), published quarterly. IJDR publishes scientific papers on well designed and controlled original research involving orodental sciences. Papers may also include reports on unusual and interesting case presentations and invited review papers on significant topics.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信