C-Kit Immunohistochemical Expression as a Complementary Method to Assess Mast Cell Density in Helicobacter pylori-Mediated Gastritis.

IF 3 3区医学 Q2 GASTROENTEROLOGY & HEPATOLOGY

Digestion Pub Date : 2024-09-13 DOI:10.1159/000541387

Ava T Ismael, Rafal A Abdulhameed, Bushra A Hamdi, Rawaz D Tawfeeq, Aram Ommar

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Abstract

Introduction: Chronic gastritis is a group of conditions commonly characterized by stomach lining inflammation. The study aimed to investigate the clinical and pathological aspects that play a role in its development. Additionally, the study examines the use of CD117 as an immunohistochemistry marker in evaluating mast cell density (MCD).

Methods: This retrospective, cross-sectional study was conducted in Iraqi Kurdistan with a sample size of 380 patients. Patient data included gastritis type, neutrophil infiltration severity, mononuclear cell infiltration within the lamina propria, intestinal metaplasia, and glandular atrophy, which were categorized and given a score. The CD117 level was identified using an anti-human rabbit polyclonal antibody.

Results: A statistically significant association was revealed between Helicobacter pylori-mediated gastritis and non-specific gastritis with age, activity, H. pylori and MCD, dysplasia, and malignancy. Meanwhile, no association was found with gender, inflammatory infiltrate, intestinal metaplasia, and glandular atrophy. C-Kit exhibited a marked increase in MCD in patients with H. pylori-mediated gastritis, intestinal metaplasia, atrophy, and gastric carcinoma. However, a significant decrease in MCD was observed on repeating endoscopy evaluations for patients after treatment.

Conclusion: Regions that exhibit severe inflammation, metaplasia, atrophy, and carcinoma demonstrated an increase in MCD with H. pylori-mediated gastritis. A detailed investigation in clinical practice to screen early diagnosis and treatment needs to be performed in high H. pylori prevalence.

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将 C-Kit 免疫组化表达作为评估幽门螺旋杆菌介导的胃炎中肥大细胞密度的辅助方法

简介慢性胃炎是一组以胃黏膜炎症为常见特征的疾病。本研究旨在探讨导致慢性胃炎的临床和病理因素。此外，该研究还探讨了使用 CD117 作为免疫组化标记来评估肥大细胞密度（MCD）：这项回顾性横断面研究在伊拉克库尔德斯坦进行，样本量为 380 例患者。患者数据包括胃炎类型、中性粒细胞浸润严重程度、固有层内单核细胞浸润、肠化生和腺体萎缩，并对其进行分类和评分。使用抗人兔多克隆抗体鉴定 CD117 水平：结果：幽门螺杆菌介导的胃炎和非特异性胃炎与年龄、活动度、幽门螺杆菌和 MCD、发育不良和恶性肿瘤有统计学意义。同时，与性别、炎症浸润、肠化生和腺体萎缩没有关联。在幽门螺杆菌介导的胃炎、肠化生、萎缩和胃癌患者中，C-Kit 显示 MCD 明显增加。然而，在治疗后对患者进行重复内镜评估时，观察到 MCD 明显下降：结论：在幽门螺杆菌介导的胃炎中，表现出严重炎症、化生、萎缩和癌变的区域的 MCD 会增加。在幽门螺杆菌高发区，需要在临床实践中进行详细调查，以筛查早期诊断和治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Digestion 医学-胃肠肝病学

CiteScore

7.90

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Digestion'' concentrates on clinical research reports: in addition to editorials and reviews, the journal features sections on Stomach/Esophagus, Bowel, Neuro-Gastroenterology, Liver/Bile, Pancreas, Metabolism/Nutrition and Gastrointestinal Oncology. Papers cover physiology in humans, metabolic studies and clinical work on the etiology, diagnosis, and therapy of human diseases. It is thus especially cut out for gastroenterologists employed in hospitals and outpatient units. Moreover, the journal''s coverage of studies on the metabolism and effects of therapeutic drugs carries considerable value for clinicians and investigators beyond the immediate field of gastroenterology.