Preparation of hapten and monoclonal antibody of hesperetin and establishment of enzyme-linked immunosorbent assay.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-01-01 Epub Date: 2024-09-18 DOI:10.1016/j.talanta.2024.126912

Yifan Liu, Zihui Jin, Di Sun, Bo Xu, Tianyu Lan, Qiyang Zhao, Yue He, Jing Li, Yongliang Cui, Yaohai Zhang

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引用次数: 0

Abstract

Hesperetin is the aglycone of hesperidin and is widely found in the Rutaceae plants and herbal medicines. It exhibits antioxidant, detoxifying, anti-inflammatory, and antimicrobial properties, similar to hesperidin. It has also shown potential in the regulation of certain diseases. In order to detect hesperetin in complex matrix samples such as citrus and herbal, we developed an anti-hesperetin monoclonal antibody and established an indirect competitive enzyme-linked immunosorbent assay (icELISA). The half maximal inhibitory concentration (IC₅₀) was determined to be 2.03 ng/mL, the detection range was 0.39-12.73 ng/mL. In practical sample testing, the concentration of hesperidin measured by icELISA is consistent with the result of UPLC-MS/MS, and the correlation coefficient (R²) is 0.97. These results showed that the established method has good accuracy, reproducibility and broad application prospects, and can be used for the detection of hesperetin in complex matrix samples (such as citrus and herbal samples).

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制备七叶皂苷及单克隆抗体并建立酶联免疫吸附试验。

橙皮甙是橙皮素的苷元，广泛存在于芸香科植物和草药中。它具有与橙皮甙类似的抗氧化、解毒、消炎和抗菌特性。它还具有调节某些疾病的潜力。为了检测柑橘和中草药等复杂基质样品中的橙皮素，我们开发了一种抗橙皮素单克隆抗体，并建立了一种间接竞争性酶联免疫吸附试验（icELISA）。经测定，半最大抑制浓度（IC50）为 2.03 ng/mL，检测范围为 0.39-12.73 ng/mL。在实际样品检测中，icELISA法测定的橙皮甙浓度与UPLC-MS/MS法测定的结果一致，相关系数（R2）为0.97。这些结果表明所建立的方法具有良好的准确性、重现性和广阔的应用前景，可用于复杂基质样品（如柑橘和中草药样品）中橙皮素的检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.

文献相关原料

公司名称	产品信息	采购帮参考价格
上海源叶	neohesperidin
上海源叶	didymin
上海源叶	naringin
上海源叶	naringenin
上海源叶	N-Hydroxysuccinimide (NHS)
上海源叶	sinensetin
上海源叶	rutin
上海源叶	rhoifolin
上海源叶	tangeretin
上海源叶	hesperidin
上海源叶	phloretin
上海源叶	narirutin