Soluble trehalase responds to heavy metal stimulation by regulating apoptosis in Neocaridina denticulata sinensis

IF 6.2 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Ecotoxicology and Environmental Safety Pub Date : 2024-09-19 DOI:10.1016/j.ecoenv.2024.117072

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Abstract

Trehalase plays an important role in insect metabolism and development by hydrolyzing blood sugar trehalose, but it seems to perform primarily an immunomodulatory function in crustaceans whose blood sugar is glucose. Metal ions as pollutants seriously affecting crustacean health, but studies on trehalase in metal immunity are still limited. In this study, a soluble trehalase (NdTre1) that could bind to multiple metals was identified from Neocaridina denticulata sinensis for investigating metal resistance. Expression profiling revealed that NdTre1 was mainly expressed in the gill and was significantly decreased following stimulation with copper (Cu²⁺) and cadmium (Cd²⁺). Transcriptomic analysis of gills revealed an increase in ecdysone synthesis after interference with NdTre1. Increased ecdysone activated the endogenous mitochondrial pathway and the mitogen activated protein kinase (MAPK) pathway to further induced apoptosis. In vitro, Escherichia coli overexpressing recombinant NdTre1 had higher survival and faster growth rates to better adapted the metal-containing medium. Overall, NdTre1 exercises an important immune function in shrimp resistance to metal stimulation by regulating apoptosis and molting. Further investigation can further explore specific response mechanisms of NdTre1 to multiple metals.

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可溶性三卤素酶通过调节中华新鲤中的细胞凋亡对重金属刺激做出反应。

颤藻糖酶通过水解血糖颤藻糖而在昆虫的新陈代谢和发育过程中发挥重要作用，但在血糖为葡萄糖的甲壳类动物中，它似乎主要发挥免疫调节功能。金属离子作为污染物严重影响甲壳类动物的健康，但有关三卤糖酶在金属免疫方面的研究仍然有限。本研究从Neocaridina denticulata sinensis中发现了一种可与多种金属结合的可溶性三卤化酶（NdTre1），用于研究金属抗性。表达谱分析显示，NdTre1主要在鳃中表达，在铜（Cu²⁺）和镉（Cd²⁺）刺激下表达量显著下降。鳃的转录组分析表明，在干扰 NdTre1 后，蜕皮激素合成增加。增加的蜕皮激素激活了内源性线粒体途径和丝裂原活化蛋白激酶（MAPK）途径，进一步诱导细胞凋亡。在体外，过表达重组 NdTre1 的大肠杆菌存活率更高，生长速度更快，能更好地适应含金属的培养基。总之，NdTre1 通过调节细胞凋亡和蜕皮，在对虾抵抗金属刺激的过程中发挥了重要的免疫功能。进一步的研究可以进一步探索 NdTre1 对多种金属的特定响应机制。

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来源期刊

Ecotoxicology and Environmental Safety 环境科学-毒理学

CiteScore

12.10

自引率

5.90%

发文量

1234

审稿时长

88 days

期刊介绍： Ecotoxicology and Environmental Safety is a multi-disciplinary journal that focuses on understanding the exposure and effects of environmental contamination on organisms including human health. The scope of the journal covers three main themes. The topics within these themes, indicated below, include (but are not limited to) the following: Ecotoxicology、Environmental Chemistry、Environmental Safety etc.