Bayesian Individual Limits for IGF-1 Monitoring in Equine Plasma: Implementation in the Equine Biological Passport.

IF 2.6 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Drug Testing and Analysis Pub Date : 2024-09-16 DOI:10.1002/dta.3795

Agnès Barnabé, Benoit Loup, Adam Cawley, Vivian Delcourt, Patrice Garcia, Marie-Agnès Popot, John Keledjian, Ludovic Bailly-Chouriberry

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引用次数: 0

Abstract

Despite the International Federation of Horseracing Authorities (IFHA) regulation associated with heavy sanctions, the abuse of prohibited substances must be identified and deterred throughout horses' athletic careers, such as the administration of recombinant growth hormone (rGH). GH is naturally produced in mammal organisms to stimulate growth. Thus, rGH administration can enhance the performance of horses by expanding some physical abilities. As measuring endogenous GH levels is complex, an indirect strategy is to monitor GH-associated biomarkers in plasma as insulin-like growth factor 1 (IGF-1) levels. To prevent these misuses, the Equine Biological Passport (EBP) has been designed in France (GIE LCH) and Australia (ARFL-Racing NSW) to profile specific biological and chemical parameters in selected racehorses. In this study, we investigated individual limits as a complementary tool to a single limit to supervise the stability of IGF-1 profile over a racing season. The aim is to design custom limits based on the horse's history to detect any deviation below the single limit. The method was assessed using experimental data and then tested on EBP data from three thoroughbreds and three French trotters. Finally, individual limits have been added to the French EBP for IGF-1 monitoring.

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马血浆中 IGF-1 监测的贝叶斯个体限值：在马生物护照中的实施。

尽管国际赛马管理机构联合会（IFHA）规定了严厉的处罚措施，但在马匹的整个运动生涯中，必须识别和阻止滥用禁用物质的行为，例如使用重组生长激素（rGH）。生长激素是哺乳动物体内自然产生的一种刺激生长的物质。因此，使用重组生长激素可以提高马匹的某些身体机能，从而提高马匹的运动表现。由于测量内源性 GH 水平非常复杂，一种间接的策略是监测血浆中与 GH 相关的生物标志物，如胰岛素样生长因子 1 (IGF-1) 水平。为了防止这些误用，法国（GIE LCH）和澳大利亚（ARFL-Racing NSW）设计了马匹生物护照（EBP），对选定赛马的特定生物和化学参数进行分析。在这项研究中，我们调查了作为单一限值补充工具的个体限值，以监督 IGF-1 在一个赛季中的稳定性。目的是根据赛马的历史记录设计定制限值，以检测任何低于单一限值的偏差。该方法利用实验数据进行了评估，然后在三匹纯血马和三匹法国小步赛马的 EBP 数据上进行了测试。最后，在法国 EBP 中添加了用于 IGF-1 监测的单个限值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Drug Testing and Analysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

5.90

自引率

24.10%

发文量

191

审稿时长

2.3 months

期刊介绍： As the incidence of drugs escalates in 21st century living, their detection and analysis have become increasingly important. Sport, the workplace, crime investigation, homeland security, the pharmaceutical industry and the environment are just some of the high profile arenas in which analytical testing has provided an important investigative tool for uncovering the presence of extraneous substances. In addition to the usual publishing fare of primary research articles, case reports and letters, Drug Testing and Analysis offers a unique combination of; ‘How to’ material such as ‘Tutorials’ and ‘Reviews’, Speculative pieces (‘Commentaries’ and ‘Perspectives'', providing a broader scientific and social context to the aspects of analytical testing), ‘Annual banned substance reviews’ (delivering a critical evaluation of the methods used in the characterization of established and newly outlawed compounds). Rather than focus on the application of a single technique, Drug Testing and Analysis employs a unique multidisciplinary approach to the field of controversial compound determination. Papers discussing chromatography, mass spectrometry, immunological approaches, 1D/2D gel electrophoresis, to name just a few select methods, are welcomed where their application is related to any of the six key topics listed below.