The improvement of immunity and activation of TLR2/NF-κB signaling pathway by Romboutsia ilealis in broilers

IF 2.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of animal science Pub Date : 2024-09-21 DOI:10.1093/jas/skae286

Bochen Song, Peng Sun, Linglian Kong, Chuanpi Xiao, Xue Pan, Zhigang Song

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引用次数: 0

Abstract

This study was conducted to investigate the effects of Romboutsia (R.) ilealis on the immune function of broilers and the underlying mechanisms. A total of 48 one-d-old Arbor Acres broilers were allocated to 4 groups as follows: broilers treated daily with 1 mL live R. ilealis in GAM broth media (0, 1×104, 1×106 and 1×108 CFU/mL) from d 1 to 7. Samples were collected on d 8 and 14. The results showed that R. ilealis had no negative effect on the body weight of broilers (P > 0.05). R. ilealis significantly increased the levels of lysozyme, IFN-γ, IFN-γ/IL-4, and IgG in the serum (P < 0.05). R. ilealis significantly increased the levels of IL-4, IFN-γ, sIgA, lysozyme, and iNOS in the ileal mucosa (P < 0.05). R. ilealis significantly increased the mRNA levels of TLR2, TLR4, NF-κB, IL-1β, TNF-α, IFN-γ, IgA, pIgR, iNOS, and MHC-Ⅱ in the ileum (P < 0.05). R. ilealis significantly increased the relative abundance of Enterococcus and Paracoccus in the jejunum and ileum, ileal Candidatus Arthromitus, and cecal Romboutsia and Intestinimonas (P < 0.05). Correlation analysis showed that Enterococcus, Paracoccus, Romboutsia, and Intestinimonas were significantly positively correlated with humoral immune function (P < 0.05). In conclusion, Romboutsia ilealis boosted the immune system, activated the intestinal TLR2/NF-κB signaling pathway, and improved the gut microbiota in broilers.

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回肠隆凸菌提高肉鸡免疫力和激活 TLR2/NF-κB 信号通路的作用

本研究旨在探讨回肠球虫对肉鸡免疫功能的影响及其内在机制。将 48 只 1 日龄 Arbor Acres 肉鸡分为以下 4 组：从第 1 天到第 7 天，每天在 GAM 肉汤培养基（0、1×104、1×106 和 1×108 CFU/mL）中添加 1 mL 活回肠球虫，第 8 天和第 14 天采集样品。结果表明，回肠球菌对肉鸡的体重没有负面影响（P&;gt; 0.05）。回肠球菌能明显提高血清中溶菌酶、IFN-γ、IFN-γ/IL-4 和 IgG 的水平（P < 0.05）。回肠球菌能明显提高回肠粘膜中 IL-4、IFN-γ、sIgA、溶菌酶和 iNOS 的水平（P &;lt;0.05）。R. ilealis 能明显提高回肠中 TLR2、TLR4、NF-κB、IL-1β、TNF-α、IFN-γ、IgA、pIgR、iNOS 和 MHC-Ⅱ 的 mRNA 水平（P < 0.05）。R. ilealis 能明显增加空肠和回肠中肠球菌和副球菌、回肠中关节念珠菌、盲肠中肠球菌和肠念珠菌的相对丰度（P &;lt;0.05）。相关分析表明，肠球菌、副球菌、隆布氏菌和肠球菌与体液免疫功能呈显著正相关（P &;lt;0.05）。总之，回肠球菌能增强肉鸡的免疫系统，激活肠道 TLR2/NF-κB 信号通路，并改善肉鸡的肠道微生物群。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of animal science 农林科学-奶制品与动物科学

CiteScore

4.80

自引率

12.10%

发文量

1589

审稿时长

3 months

期刊介绍： The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.