The Influence of Removable Complete Denture on Pro-Oxidant Antioxidant Balance and Redox-Sensitive Inflammation Biomarker NF-ĸB in the Oral Cavity: An Interventional Follow-Up Study

IF 1.7 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical and Experimental Dental Research Pub Date : 2024-09-19 DOI:10.1002/cre2.70007

Mirjana Bošković, Dušan Sokolović, Saša Stanković, Ivan Ristić, Jordan Popović, Gordana Kocić

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Abstract

Objectives

Oxidative stress, an imbalance between the body's natural antioxidant defenses and the production of reactive oxygen species (ROS), can result in serious oral diseases, including oral cancer, periodontal diseases, and oral lichen planus, through the activation of the redox-sensitive transcription factors and inflammation. The purpose of this study was to assess the potential effects of a removable complete denture on the levels of oxidative stress markers, such as lipid peroxidation (MDA), advanced oxidation protein products (AOPP), and catalase, and the quantitative expression of the redox-sensitive transcription factor NF-κB p65 subunit.

Materials and Methods

This interventional follow-up study enrolled 40 participants of both sexes aged 28–78 years, with a median age of 56 years, where unstimulated saliva was collected before denture placement, immediately after the denture placement, and 24 h, 7 days, and 30 days after the denture placement. The most prominent ROS overproduction was reported on the seventh day (p < 0.05), followed by a significant fall in antioxidative defense.

Results

The NF-κB p65 subunit, whose expression pattern was highest in the same time period on the seventh day, serves as a signaling molecule for redox imbalance due to ROS production. Over the next 30 days, its levels remained moderately increased compared to the basal value, which may influence pro-inflammatory pathways and the integrity of oral tissue components. These alterations may be induced by the dentures, which can produce high pressures on the supporting tissues or by the synthetic materials used for producing the dentures.

Conclusion

Our research may help to clarify the potential pathways by which oxidative stress and redox-sensitive inflammatory mediators, as well as mechanical and chemical irritants, may serve as risk factors for premalignant lesions in the mouth. Further research on this topic is required to understand the molecular mechanisms behind the relationship between inflammation and oral premalignant lesions caused by mechanical and chemical irritation.

Abstract Image

查看原文本刊更多论文

可摘全口义齿对口腔中促氧化剂抗氧化剂平衡和氧化还原敏感性炎症生物标志物 NF-ĸB 的影响：干预性随访研究

目的氧化应激是人体天然抗氧化防御系统与活性氧（ROS）产生之间的失衡，可通过激活氧化还原敏感转录因子和炎症而导致严重的口腔疾病，包括口腔癌、牙周病和口腔扁平苔藓。本研究的目的是评估可摘全口义齿对氧化应激标志物（如脂质过氧化物（MDA）、高级氧化蛋白产物（AOPP）和过氧化氢酶）水平的潜在影响，以及对氧化还原反应敏感的转录因子 NF-κB p65 亚基的定量表达。材料与方法这项干预性随访研究共纳入 40 名参与者，男女不限，年龄在 28-78 岁之间，中位年龄为 56 岁，研究人员在义齿安装前、义齿安装后立即、义齿安装后 24 小时、7 天和 30 天收集未刺激唾液。第七天时，ROS 的过量产生最为明显（p <0.05），随后抗氧化防御能力显著下降。结果 NF-κB p65 亚基的表达模式在第七天的同一时段最高，它是 ROS 产生导致氧化还原失衡的信号分子。在接下来的 30 天中，其水平与基础值相比保持适度增加，这可能会影响促炎途径和口腔组织成分的完整性。这些变化可能是由假牙引起的，因为假牙会对支持组织产生高压力，也可能是由制作假牙的合成材料引起的。结论我们的研究可能有助于阐明氧化应激和氧化还原敏感性炎症介质以及机械和化学刺激物可能成为口腔癌前病变风险因素的潜在途径。要了解炎症与机械和化学刺激引起的口腔恶性病变之间关系背后的分子机制，还需要对这一课题进行进一步的研究。

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来源期刊

Clinical and Experimental Dental Research DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.30

自引率

5.60%

发文量

165

审稿时长

26 weeks

期刊介绍： Clinical and Experimental Dental Research aims to provide open access peer-reviewed publications of high scientific quality representing original clinical, diagnostic or experimental work within all disciplines and fields of oral medicine and dentistry. The scope of Clinical and Experimental Dental Research comprises original research material on the anatomy, physiology and pathology of oro-facial, oro-pharyngeal and maxillofacial tissues, and functions and dysfunctions within the stomatognathic system, and the epidemiology, aetiology, prevention, diagnosis, prognosis and therapy of diseases and conditions that have an effect on the homeostasis of the mouth, jaws, and closely associated structures, as well as the healing and regeneration and the clinical aspects of replacement of hard and soft tissues with biomaterials, and the rehabilitation of stomatognathic functions. Studies that bring new knowledge on how to advance health on the individual or public health levels, including interactions between oral and general health and ill-health are welcome.