Impact of salinity on Streptococcus agalactiae and health parameters of Oreochromis niloticus during streptococcosis outbreaks

The Microbe Pub Date : 2024-09-16 DOI:10.1016/j.microb.2024.100167

Rajan Preenanka , Muhammed P. Safeena , Basura Vidhya , TG Sumithra

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Abstract

S. agalactiae infection is one of the most important bacterial diseases imparting mass mortality in the aquaculture sector, worldwide. It is responsible for posing both economic burdens and risks of cross-infectivity and zoonotic transmission. In this aspect, this study examined the virulence gene profiles, molecular serotyping and pathogenicity of S. agalactiae strains isolated from tilapia farms in Kerala, India in 2022. Following sample collection and processing, three S. agalactiae strains (KSA/01, KSA/02, and KSA/03) were isolated and confirmed the bacterial identity using 16S rRNA sequencing. In pathogenicity test, the LD₅₀ of the S. agalactiae was found to be 1 × 10⁶ CFU/ml. An increase in the salinity was found to affect the pathogenicity of S. agalactiae as well as the hematological and serological profile of tilapia. Thus the presence of S. agalactiae, a highly virulent and zoonotic pathogen in tilapia farms, underscores the imperative of continuous monitoring in aquaculture facilities.

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在链球菌病爆发期间，盐度对无乳链球菌和黑鲷健康参数的影响

在全球范围内，水产养殖业中造成大量死亡的最重要细菌性疾病之一就是无乳猪繁殖病（S. agalactiae）感染。它既造成经济负担，又有交叉感染和人畜共患病传播的风险。为此，本研究检测了 2022 年从印度喀拉拉邦罗非鱼养殖场分离出的 S. agalactiae 菌株的毒力基因图谱、分子血清分型和致病性。样品采集和处理后，分离出三株 S. agalactiae 菌株（KSA/01、KSA/02 和 KSA/03），并通过 16S rRNA 测序确认了细菌身份。在致病性试验中，发现 S. agalactiae 的半数致死剂量为 1 × 106 CFU/ml。盐度的增加会影响 S. agalactiae 的致病性以及罗非鱼的血液学和血清学特征。因此，罗非鱼养殖场中存在的高致病性人畜共患病病原体 S. agalactiae 强调了对水产养殖设施进行持续监测的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Microbe

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