Development and evaluation of a loop-mediated isothermal amplification (LAMP) method for Candida glabrata detection

Abstract

Purpose

Loop-mediated isothermal amplification (LAMP) is a simple and rapid nucleic acid method for DNA amplification at a constant temperature. The “gold standard” culture method for yeast detection, has low sensitivity with severe consequences, increasing morbidity and mortality rates. Here, we report the development of a LAMP method for the specific detection of C. glabrata.

Methodology

The specific LAMP primers for C. glabrata detection were designed and evaluated.

Results

The LAMP assay accurately detected C. glabrata with no cross-reactivity with other Candida species.

Conclusion

The developed molecular method would be a promising tool in the management of invasive candidiasis.