Validity of reducing blood culture incubation time for the BD BACTEC FX blood culture system considering microbiological and clinical aspects

IF 1.9 4区医学 Q3 INFECTIOUS DISEASES

Journal of Infection and Chemotherapy Pub Date : 2024-09-12 DOI:10.1016/j.jiac.2024.09.011

Takehiro Hashimoto, Takaaki Yahiro, Sakirul Khan, Kazunori Kimitsuki, Akira Nishizono, Kazufumi Hiramatsu

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引用次数: 0

Abstract

A blood culture (BC) test is vital for diagnosing bacteremia in clinical practice. Although incubation time varies among automated BC systems, 4–5 days is deemed to be sufficient time for the BD BACTEC FX blood culture system. This study compared the clinical and microbiological characteristics of true-positive BC samples on day 5 with those within 4 days to reduce missed true bacteremia cases. We conducted a retrospective study of blood cultures from hospitalized patients between April 2020 and April 2023 at a tertiary care hospital in Japan. In total, 12,342 BC sets were collected from 6,567 patients. Gram-positive bacilli other than spp. and spp., non-, and yeasts other than spp. were detected more frequently in BC-positive patients on day 5 than in those within 4 days. The gastrointestinal tract was the portal of entry more frequently on day 5 than within 4 days (25 % vs. 4 %, p = 0.006). A 4-day incubation period is sufficient for the BD BACTEC FX blood culture system under routine conditions. However, a 5-day incubation period may be warranted when low pathogenicity is suspected or the gastrointestinal tract is suspected as the portal of entry.

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从微生物学和临床角度考虑缩短 BD BACTEC FX 血液培养系统培养时间的有效性

在临床实践中，血液培养（BC）检测对诊断菌血症至关重要。尽管自动 BC 系统的培养时间各不相同，但对于 BD BACTEC FX 血培养系统来说，4-5 天被认为是足够长的时间。本研究比较了第 5 天和 4 天内真正阳性 BC 样本的临床和微生物学特征，以减少真正菌血症病例的漏诊。我们对日本一家三级医院 2020 年 4 月至 2023 年 4 月期间住院患者的血培养结果进行了回顾性研究。总共从 6567 名患者中收集了 12342 套 BC。在 BC 阳性患者中，第 5 天检测到的革兰氏阳性杆菌（非属和属）、非和酵母菌（非属和属）的频率高于 4 天内的患者。胃肠道在第 5 天比 4 天内更常成为感染入口（25% 对 4%，P = 0.006）。在常规条件下，BD BACTEC FX 血液培养系统的培养期为 4 天就足够了。但是，如果怀疑病原体的致病性较低或怀疑病原体是从胃肠道进入的，则需要 5 天的潜伏期。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Infection and Chemotherapy INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY

CiteScore

4.10

自引率

4.50%

发文量

303

审稿时长

47 days

期刊介绍： The Journal of Infection and Chemotherapy (JIC) — official journal of the Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases — welcomes original papers, laboratory or clinical, as well as case reports, notes, committee reports, surveillance and guidelines from all parts of the world on all aspects of chemotherapy, covering the pathogenesis, diagnosis, treatment, and control of infection, including treatment with anticancer drugs. Experimental studies on animal models and pharmacokinetics, and reports on epidemiology and clinical trials are particularly welcome.