P. Kastelein, A. Evenhuis, M. C. Krijger, J. M. van der Wolf
{"title":"Aerial dispersion of Xanthomonas fragariae during trimming leaves of angular leaf spot diseased strawberry propagation plants","authors":"P. Kastelein, A. Evenhuis, M. C. Krijger, J. M. van der Wolf","doi":"10.1007/s10658-024-02950-w","DOIUrl":null,"url":null,"abstract":"<p>The bacterium <i>Xanthomonas fragariae</i> is the causative agent of angular leaf spot (ALS) of strawberry, a Regulated Non-Quarantine Pest in Europe (EPPO A2) for plant propagation material. Field experiments were conducted to explore if <i>X. fragariae</i> is dispersed through the air during trimming ALS-diseased strawberry plants with dry or wetted leaves. Trimming the leaves led to dissemination of leaf fragments to the nearby surroundings. A sharp decrease in the amount of leaf fragments within the first 5 m distance downwind from the strawberry plants was found. Furthermore, air quality monitors demonstrated that during trimming 0.5 – 10 μm sized particles were ejected into the air, resulting in short periods with increased particle densities 45 cm above ground level, which could be detected at least 50 m downwind. At this height <i>X. fragariae</i> was detected by means of air samplers, as evidenced with a combination of dilution-plating and TaqMan assays, at 25 m distance downwind from ALS-diseased plants. A sharp decrease in the density of <i>X. fragariae</i> colony forming units (cfu) within the first 10 m distance from the source plants was found. The densities of <i>X. fragariae</i> cfu were strongly associated with the particle densities. Results indicate that during trimming leaves of strawberry propagation crops with ALS-diseased plants there is a considerable risk of deposition of airborne <i>X. fragariae</i> inoculum on nearby nursery beds. Whether this airborne inoculum can result in infections is discussed.</p>","PeriodicalId":12052,"journal":{"name":"European Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10658-024-02950-w","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
Abstract
The bacterium Xanthomonas fragariae is the causative agent of angular leaf spot (ALS) of strawberry, a Regulated Non-Quarantine Pest in Europe (EPPO A2) for plant propagation material. Field experiments were conducted to explore if X. fragariae is dispersed through the air during trimming ALS-diseased strawberry plants with dry or wetted leaves. Trimming the leaves led to dissemination of leaf fragments to the nearby surroundings. A sharp decrease in the amount of leaf fragments within the first 5 m distance downwind from the strawberry plants was found. Furthermore, air quality monitors demonstrated that during trimming 0.5 – 10 μm sized particles were ejected into the air, resulting in short periods with increased particle densities 45 cm above ground level, which could be detected at least 50 m downwind. At this height X. fragariae was detected by means of air samplers, as evidenced with a combination of dilution-plating and TaqMan assays, at 25 m distance downwind from ALS-diseased plants. A sharp decrease in the density of X. fragariae colony forming units (cfu) within the first 10 m distance from the source plants was found. The densities of X. fragariae cfu were strongly associated with the particle densities. Results indicate that during trimming leaves of strawberry propagation crops with ALS-diseased plants there is a considerable risk of deposition of airborne X. fragariae inoculum on nearby nursery beds. Whether this airborne inoculum can result in infections is discussed.
期刊介绍:
The European Journal of Plant Pathology is an international journal publishing original articles in English dealing with fundamental and applied aspects of plant pathology; considering disease in agricultural and horticultural crops, forestry, and in natural plant populations. The types of articles published are :Original Research at the molecular, physiological, whole-plant and population levels; Mini-reviews on topics which are timely and of global rather than national or regional significance; Short Communications for important research findings that can be presented in an abbreviated format; and Letters-to-the-Editor, where these raise issues related to articles previously published in the journal. Submissions relating to disease vector biology and integrated crop protection are welcome. However, routine screenings of plant protection products, varietal trials for disease resistance, and biological control agents are not published in the journal unless framed in the context of strategic approaches to disease management.