Bioprospecting of wild botanicals against alternaria leaf blight of radish and their phytochemical profiling by GC–MS

IF 1.7 3区 农林科学 Q2 AGRONOMY
Ranjan Kumar, T. Rajesh, Sudharshan Keralapura Ramachandra, Snata Kaushik, Hans Austin K H
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Abstract

Radish, considered a globally important crop, faces a severe threat from Alternaria blight. This pervasive and highly damaging disease causes black spots and blight on leaves in field conditions, resulting in reduced yield and seed quality. The study aimed to evaluate the antifungal potential of different wild botanicals against the Alternaria leaf blight pathogen through in vitro and in vivo studies and to analyze the phytochemicals using Gas Chromatography-Mass Spectrometry (GC–MS).

Among the fifty botanicals screened at 10% concentration against the pathogen, the aqueous extracts of Hemidesmus indicus, Lippia alba, Chromolaena odorata, and Solanum violaceum displayed the highest mycelial inhibition. Further in vitro evaluations of these four botanicals at different concentrations (2.5%, 5.0%, 7.5%, 10.0%, and 12.5%) revealed that at a 12.5% plant extract concentration H. indicus and L. alba exhibited the most effective inhibition of 82.96% and 76.60%, respectively, followed by C. odorata (71.93%) and S. violaceum (63.53%). Based on the promising in vitro results, these botanicals were assessed in pot experiments at 12.5% concentration. Among the four botanicals, the highest percentage of disease reduction was observed with H. indicus and L. alba, with 69.79% and 60.44%, respectively. Overall, the botanicals effectively reduced disease severity while increasing radish yield. GC–MS analysis of the phytochemicals revealed that the botanicals are composed of various bioactive compounds such as phenolics, flavonoids, saponins, and volatile compounds, which are known to exhibit antimicrobial activities.

This study emphasizes the potential of botanicals as a natural alternative for managing fungal diseases, offering resilient and sustainable approaches to safeguard crops from destructive fungal infections.

Abstract Image

针对萝卜交替孢霉叶枯病的野生植物生物勘探及其植物化学成分的 GC-MS 分析
萝卜被认为是全球重要的农作物,但却面临着 Alternaria 枯萎病的严重威胁。这种普遍存在、危害性极大的病害会在田间造成叶片黑斑和枯萎,导致产量和种子质量下降。该研究旨在通过体外和体内研究,评估不同野生植物对 Alternaria 叶枯病病原体的抗真菌潜力,并使用气相色谱-质谱法(GC-MS)分析植物化学物质。在筛选出的 50 种浓度为 10%的植物中,Hemidesmus indicus、Lippia alba、Chromolaena odorata 和 Solanum violaceum 的水提取物对病原体的菌丝抑制作用最强。对这四种不同浓度(2.5%、5.0%、7.5%、10.0% 和 12.5%)的植物提取物进行的进一步体外评估显示,在 12.5% 的植物提取物浓度下,半枝莲和白花蛇舌草的抑制效果最好,分别为 82.96% 和 76.60%,其次是臭椿(71.93%)和茄属(63.53%)。基于良好的体外实验结果,我们在盆栽实验中以 12.5% 的浓度对这些植物药进行了评估。在这四种植物药中,H. indicus 和 L. alba 对病害的抑制率最高,分别为 69.79% 和 60.44%。总体而言,这些植物药在提高萝卜产量的同时有效降低了病害的严重程度。植物化学物质的气相色谱-质谱分析表明,植物药由多种生物活性化合物组成,如酚类、黄酮类、皂苷和挥发性化合物,这些化合物具有抗菌活性。
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来源期刊
European Journal of Plant Pathology
European Journal of Plant Pathology 农林科学-农艺学
CiteScore
4.20
自引率
5.60%
发文量
183
审稿时长
3 months
期刊介绍: The European Journal of Plant Pathology is an international journal publishing original articles in English dealing with fundamental and applied aspects of plant pathology; considering disease in agricultural and horticultural crops, forestry, and in natural plant populations. The types of articles published are :Original Research at the molecular, physiological, whole-plant and population levels; Mini-reviews on topics which are timely and of global rather than national or regional significance; Short Communications for important research findings that can be presented in an abbreviated format; and Letters-to-the-Editor, where these raise issues related to articles previously published in the journal. Submissions relating to disease vector biology and integrated crop protection are welcome. However, routine screenings of plant protection products, varietal trials for disease resistance, and biological control agents are not published in the journal unless framed in the context of strategic approaches to disease management.
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