HOXA9 promotes proliferation, metastasis and prevents apoptosis in hepatocellular carcinoma

IF 2.7 3区 医学 Q3 ONCOLOGY
Guojian Bao, Haowei Wei, Jiawu Yan, Yunzheng Li, Cailin Xue, Rao Fu, Minglu Zhang, Jialu Ding, Hengqian He, Decai Yu, Fei Yang, Beicheng Sun
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Abstract

Purpose

This research aimed to evaluate the expression level of Homeobox A9 (HOXA9) and its role in tumorigenesis of hepatocellular carcinoma (HCC).

Methods

Bioinformatic analysis, qPCR and Western blot analysis of clinical samples were employed to evaluate mRNA and protein levels of HOXA9 in HCC patients and cell lines. In vitro cell proliferation, migration and invasion, cloning formation, xenograft tumor model, wound healing and apoptosis assays, RNA sequencing analysis of RPL38-silenced HCC-LM3 cells and qPCR, Western blot analysis were performed for validation. Analysis of HOXA9-related genes were conducted to identify their relationships between HOXA9.

Results

HOXA9 is dramatically upregulated in HCC. Upregulation of HOXA9 in HCC predicts poor survival of patients. Besides, HOXA9 promotes proliferation, metastasis and prevents apoptosis in HCC in vitro. In addition, HOXA9 controlled by Ribosomal protein RPL38 is upregulated in HCC. Bioinformatic analysis also indicated that HOXA9 is involved in the regulation of DNA methylation and immune infiltration in HCC.

Conclusion

HOXA9 is dramatically upregulated in hepatocellular carcinoma and predicts poor prognosis. Besides, HOXA9 promoted proliferation and metastasis and prevented apoptosis in vitro, which is regulated by Ribosomal protein RPL38 in HCC.

Abstract Image

HOXA9 促进肝细胞癌的增殖、转移并阻止其凋亡
方法采用生物信息学分析、qPCR和Western印迹分析临床样本,评估HCC患者和细胞系中HOXA9的mRNA和蛋白水平。体外细胞增殖、迁移和侵袭、克隆形成、异种移植肿瘤模型、伤口愈合和细胞凋亡试验、RPL38沉默的HCC-LM3细胞的RNA测序分析以及qPCR和Western印迹分析均用于验证。结果HOXA9在HCC中显著上调。HOXA9在HCC中显著上调,预示着患者的生存率较低。此外,HOXA9 在体外促进 HCC 的增殖、转移并阻止其凋亡。此外,HOXA9 受核糖体蛋白 RPL38 的控制,在 HCC 中上调。结论HOXA9在肝细胞癌中显著上调,可预测不良预后。此外,HOXA9 在体外促进增殖和转移,阻止细胞凋亡,这在 HCC 中受核糖体蛋白 RPL38 的调控。
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来源期刊
CiteScore
4.00
自引率
2.80%
发文量
577
审稿时长
2 months
期刊介绍: The "Journal of Cancer Research and Clinical Oncology" publishes significant and up-to-date articles within the fields of experimental and clinical oncology. The journal, which is chiefly devoted to Original papers, also includes Reviews as well as Editorials and Guest editorials on current, controversial topics. The section Letters to the editors provides a forum for a rapid exchange of comments and information concerning previously published papers and topics of current interest. Meeting reports provide current information on the latest results presented at important congresses. The following fields are covered: carcinogenesis - etiology, mechanisms; molecular biology; recent developments in tumor therapy; general diagnosis; laboratory diagnosis; diagnostic and experimental pathology; oncologic surgery; and epidemiology.
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