MeJA Changes Root Growth, Iridoid, Xanthone, and Secoiridoid Production, as well as Gene Expression Levels in Root Cultures of Endangered Gentiana lutea and Gentiana boissieri

IF 3.9 3区 生物学 Q1 PLANT SCIENCES
Ayşe Gülden Aday Kaya, İlknur Albayrak, Tunahan Demirci, Hikmet Deveci, Nilgün Göktürk Baydar
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引用次数: 0

Abstract

This study was carried out to understand the effect of MeJA on root growth, in vitro production of some bioactive compounds, and expression levels of the 1-Deoxy-D-xylulose 5-phosphate reductoisomerase (DXR), secologanin synthase (SLS), 3-hydroxy-3-methylglutaryl-CoA (HMGR), and geranyl diphosphate synthase (GPPS) genes in root cultures of endangered Gentiana lutea and endemic Gentiana boissieri. For this purpose, MeJA at different concentrations (0.1, 0.5, and 1.0 mM) was applied to the one-week-old root cultures for 2, 4, 6, 8, and 10 days. After harvest, roots were evaluated in terms of root growth criteria and contents of loganic acid (iridoid), isogentisin (xanthone), swertiamarin, sweroside, amarogentin, and gentiopicroside (secoiridoids). In the study, the effects of MeJA applications on the activities of the DXR, SLS, HMGR, and GPPS genes, believed to be responsible for iridoid, xanthone, and secoiridoid synthesis, were also investigated. It was determined that all the criteria examined significantly changed depending on MeJA concentration and harvest time. Regarding metabolite contents, the most appropriate applications were found to be 0.5 mM and 1.0 mM MeJA for 8 days for G. lutea and 0.5 mM MeJA for 4 days for G. boissieri. Additionally, the expression levels of the genes were found to change with MeJA treatments, but it was noted that establishing a linear relationship with a single substance was not possible. Another noteworthy result was that the accumulation of loganic acid, swertiamarin, sweroside, and gentiopicroside was higher in the roots of G. boissieri than in those of G. lutea. In conclusion, it was determined that in vitro production of iridoid, xanthone, and secoiridoids with significant pharmaceutical effects in Gentiana root cultures could be increased with MeJA applications. The study also revealed the need for more detailed research, including genomic analyses of Gentiana species and identification of genes associated with metabolite biosynthesis.

Abstract Image

MeJA 能改变濒危龙胆草(Gentiana lutea)和龙胆草(Gentiana boissieri)根培养物的根生长、类铱、黄酮和琥珀酰甙的产生以及基因表达水平
本研究旨在了解 MeJA 对濒危 Gentiana lutea 和地方性 Gentiana boissieri 根部培养物中根的生长、一些生物活性化合物的体外生产以及 1-脱氧-D-木酮糖 5-磷酸还原异构酶(DXR)、翘果苷合成酶(SLS)、3-羟基-3-甲基戊二酰-CoA(HMGR)和香叶醇二磷酸合成酶(GPPS)基因表达水平的影响。为此,将不同浓度(0.1、0.5 和 1.0 mM)的 MeJA 分别施加到一周龄的根培养物上 2、4、6、8 和 10 天。收获后,根据根的生长标准和络石酸(鸢尾甙)、异龙胆素(黄酮)、獐牙菜甙、獐牙菜甙、金刚甙和龙胆草甙(仲呋喃糖甙)的含量对根进行评估。在这项研究中,还调查了施用 MeJA 对 DXR、SLS、HMGR 和 GPPS 基因活性的影响。结果表明,根据 MeJA 浓度和收获时间的不同,所有检测标准都发生了显著变化。在代谢物含量方面,发现最合适的应用是:对 G. lutea 采用 0.5 mM 和 1.0 mM MeJA,持续 8 天;对 G. boissieri 采用 0.5 mM MeJA,持续 4 天。此外,还发现基因的表达水平随 MeJA 处理而变化,但注意到不可能与单一物质建立线性关系。另一个值得注意的结果是,在 G. boissieri 的根中,loganic 酸、獐牙菜苷、獐牙菜甙和龙胆草甙的积累量高于 G. lutea。总之,研究发现,应用 MeJA 可以提高龙胆草根培养物中具有显著药用效果的鸢尾酮、黄酮和琥珀酰糖苷的体外产量。该研究还揭示了进行更详细研究的必要性,包括龙胆属植物的基因组分析和与代谢物生物合成相关的基因鉴定。
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来源期刊
CiteScore
8.40
自引率
6.20%
发文量
312
审稿时长
1.8 months
期刊介绍: The Journal of Plant Growth Regulation is an international publication featuring original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research on various aspects of plant growth and development using hormonal, physiological, environmental, genetic, biophysical, developmental and/or molecular approaches. The journal also publishes timely reviews on highly relevant areas and/or studies in plant growth and development, including interdisciplinary work with an emphasis on plant growth, plant hormones and plant pathology or abiotic stress. In addition, the journal features occasional thematic issues with special guest editors, as well as brief communications describing novel techniques and meeting reports. The journal is unlikely to accept manuscripts that are purely descriptive in nature or reports work with simple tissue culture without attempting to investigate the underlying mechanisms of plant growth regulation, those that focus exclusively on microbial communities, or deal with the (elicitation by plant hormones of) synthesis of secondary metabolites.
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