Chemiluminescence detection of kanamycin by DNA aptamer regulating peroxidase-like activity of Co3O4 nanoparticles.

IF 1.8 4区 化学 Q3 CHEMISTRY, ANALYTICAL
Xuxin Zhang,Yihao Li,Shaojie Xia,Zhenyuan Yang,Baiyun Zhang,Yonghong Wang
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Abstract

Kanamycin (KAN) is widely used as a growth hormone analog and an antibacterial agent. However, abuse of this substance has resulted in the accumulation of excessive residue levels in foods of animal origin, which presents a significant risk to human health. A chemiluminescent aptasensor was constructed for the rapid quantitative detection of KAN by combining the properties of Co3O4 nanoparticles (Co3O4 NPs) nanozyme activity and DNA aptamer with high specificity. The DNA aptamer/Co3O4 NPs nanozyme regulated the chemiluminescence signal by exploiting the chemiluminescent properties of luminol oxidation by H2O2. Specific binding of KAN to the aptamer led to the formation of a steric hindrance block in the solution, which inhibited the activity of nanozyme and reduced signal intensity. The degree of signal reduction is related to the concentration of KAN. Under optimal conditions, there was good linearity between KAN concentration and chemiluminescence signal intensity in the range of 0.5-8.0 μΜ, with a detection limit of 0.26 μΜ. The detection system performed well in the presence of competing antibiotics and was virtually unaffected. The method was also suitable for the detection of KAN in milk samples with sample recoveries of 97.8%-99.1%. The chemiluminescence sensor has the advantages of low cost, specificity, and sensitivity, and does not require an external light source or modification of the nucleic acid aptamer which makes it a promising candidate for applications in the field of food detection.
通过 DNA 配体调节 Co3O4 纳米粒子的过氧化物酶样活性对卡那霉素进行化学发光检测。
卡那霉素(KAN)被广泛用作生长激素类似物和抗菌剂。然而,该物质的滥用导致动物源性食品中的残留量超标,给人类健康带来了巨大风险。本研究结合 Co3O4 纳米粒子(Co3O4 NPs)纳米酶活性和高特异性 DNA 类似物的特性,构建了一种用于快速定量检测 KAN 的化学发光类似物传感器。DNA aptamer/Co3O4 NPs 纳米酶利用发光酚被 H2O2 氧化的化学发光特性来调节化学发光信号。KAN 与适配体的特异性结合导致在溶液中形成一个立体阻碍块,从而抑制了纳米酶的活性并降低了信号强度。信号降低的程度与 KAN 的浓度有关。在最佳条件下,KAN 浓度与化学发光信号强度在 0.5-8.0 μΜ 范围内呈良好的线性关系,检测限为 0.26 μΜ。该检测系统在有竞争性抗生素存在的情况下表现良好,几乎不受影响。该方法还适用于检测牛奶样品中的 KAN,样品回收率为 97.8%-99.1%。该化学发光传感器具有成本低、特异性强、灵敏度高的优点,而且不需要外部光源或对核酸适配体进行修饰,因此有望应用于食品检测领域。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Analytical Sciences
Analytical Sciences 化学-分析化学
CiteScore
2.90
自引率
18.80%
发文量
232
审稿时长
1 months
期刊介绍: Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.
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