Box-Behnken Design for DPPH Free Radical Scavenging Activity Optimization from Microwave-Assisted Extraction of Polyphenolic Compounds from Agave lechuguilla Torr. Residues

IF 2.8 4区工程技术 Q2 ENGINEERING, CHEMICAL

Processes Pub Date : 2024-09-18 DOI:10.3390/pr12092005

Vianey de J. Cervantes-Güicho, Ana G. Reyes, Alberto Nuncio, Leonardo Sepúlveda-Torre, Cristina Landa-Cansigno, José A. Rodríguez-De la Garza, Miguel A. Medina-Morales, Leopoldo J. Ríos-González, Thelma K. Morales-Martínez

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Abstract

The guishe is a by-product of the fiber extraction from Agave lechuguilla. This material has no commercial value, although it contains metabolites that could be used as a resource for producing high-value products. This study optimized the DPPH• (2,2-diphenyl-1-picrylhydrazyl) antioxidant activity through microwave-assisted extraction (MAE) of polyphenolic compounds from Agave lechuguilla residues. The MAE process was optimized using a Box-Behnken design, with extraction time (5–15 min), temperature (40–50 °C), and solvent: sample ratio (1:20–1:30 m/v) as independent variables. In contrast, the dependent variable was DPPH• free radical scavenging activity. As a result, the highest antioxidant activity was at 8 min of irradiation, extraction temperature of 45 °C, and solvent: sample ratio 1:30 w/v, obtaining a total flavonoid content of 19.25 ± 0.60 mg QE/g DW, a total polyphenol content of 6.59 ± 0.31 mg GAE/g DW, a DPPH• free radical scavenging activity of 73.35 ± 1.90%, and an ABTS+• ([2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate)]) free radical scavenging activity of 91.93 ± 0.68%.

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微波辅助萃取龙舌兰多酚类化合物的 DPPH 自由基清除活性优化箱-贝肯设计（Box-Behnken Design for DPPH Free Radical Scavenging Activity Optimization from Microwave-Assisted Extraction of Polyphenolic Compounds from Agave lechuguilla Torr.残留物

Guishe 是龙舌兰提取纤维的副产品。这种材料没有商业价值，但它含有的代谢物可用作生产高价值产品的资源。本研究通过微波辅助萃取（MAE）优化了龙舌兰残渣中多酚化合物的 DPPH（2,2-二苯基-1-苦基肼）抗氧化活性。萃取时间（5-15 分钟）、温度（40-50 °C）和溶剂与样品的比例（1:20-1:30 m/v）是自变量。因变量则是 DPPH 自由基清除活性。结果表明，在辐照 8 分钟、提取温度为 45 °C、溶剂与样品的比例为 1:30 w/v 时，抗氧化活性最高，总黄酮含量为 19.25 ± 0.60 mg QE/g DW，总多酚含量为 6.59 ± 0.31 mg GAE/g DW，DPPH- 自由基清除活性为 73.35 ± 1.90%，ABTS+-（[2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate)]）自由基清除活性为 91.93 ± 0.68%。

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来源期刊

Processes Chemical Engineering-Bioengineering

CiteScore

5.10

自引率

11.40%

发文量

2239

审稿时长

14.11 days

期刊介绍： Processes (ISSN 2227-9717) provides an advanced forum for process related research in chemistry, biology and allied engineering fields. The journal publishes regular research papers, communications, letters, short notes and reviews. Our aim is to encourage researchers to publish their experimental, theoretical and computational results in as much detail as necessary. There is no restriction on paper length or number of figures and tables.

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