Statherin-derived peptide protects against intrinsic erosive enamel wear in situ.

IF 2.9 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Caries Research Pub Date : 2024-09-12 DOI:10.1159/000541403

Even Akemi Taira,Carolina Ruis Ferrari,Gabriel Carvalho,Vinicius Taioqui Pelá,Talita Mendes Oliveira Ventura,Edson Crusca,Reinaldo Marchetto,Marília Afonso Rabelo Buzalaf

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Abstract

INTRODUCTION This in situ study investigated the protective effect of a solution containing statherin-derived peptide (StatpSpS) against enamel intrinsic erosion. METHODS Fifteen volunteers wore appliances containing 2 bovine specimens. The samples were subjected to erosion (ERO) with HCl, mimicking dental erosion by intrinsic acid. The volunteers participated in 3 phases (double-blind and crossover): 1) Deionized water (negative control); 2) Commercial solution containing SnCl2/NaF/AmF (800 ppm Sn+2, 500 ppm F-, pH 4.5) - (positive control); 3) Solution containing 1.88 X 10-5M StatpSpS. Four times a day, the volunteers administered one drop of the solutions (50 µl, 1 min) on each specimen. After the treatment, erosive challenges were performed extraorally with 0.01 M HCl (pH 2.0, 4 times/day, 1 min, 150 mL). Enamel wear was assessed by profilometry. Data was analyzed by one-way RM-ANOVA/Bonferroni's tests (p<0.05). RESULTS In terms of the treatments, both the commercial solution - SnCl2/NaF/AmF and StatpSpS significantly reduced the wear when compared to the negative control (p<0.01), without significant differences between them (p>0.05). CONCLUSION The solution containing StatpSpS demonstrated protection against enamel intrinsic erosive wear. This study marks a significant advancement in the prevention of intrinsic erosive wear, utilizing StatpSpS in acquired pellicle engineering procedures.

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源于 Statherin 的肽可防止珐琅质的原位侵蚀性磨损。

简介这项原位研究调查了含有石炭酸肽（StatpSpS）的溶液对牙釉质内在侵蚀的保护作用。样品受到盐酸侵蚀（ERO），模拟牙齿受到内在酸的侵蚀。志愿者参与了 3 个阶段（双盲和交叉）：1）去离子水（阴性对照）；2）含 SnCl2/NaF/AmF 的商业溶液（800 ppm Sn+2，500 ppm F-，pH 4.5）-（阳性对照）；3）含 1.88 X 10-5M StatpSpS 的溶液。每天四次，志愿者在每个样本上滴一滴溶液（50 微升，1 分钟）。治疗后，用 0.01 M HCl（pH 值为 2.0，每天 4 次，1 分钟，150 毫升）在口外进行侵蚀性挑战。釉质磨损通过轮廓仪进行评估。结果与阴性对照组相比，商业溶液 - SnCl2/NaF/AmF 和 StatpSpS 均能显著减少磨损（p<0.01），两者之间无显著差异（p>0.05）。这项研究标志着在获得性颗粒工程程序中使用 StatpSpS 在防止内在侵蚀性磨损方面取得了重大进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Caries Research 医学-牙科与口腔外科

CiteScore

6.30

自引率

7.10%

发文量

审稿时长

6-12 weeks

期刊介绍： ''Caries Research'' publishes epidemiological, clinical and laboratory studies in dental caries, erosion and related dental diseases. Some studies build on the considerable advances already made in caries prevention, e.g. through fluoride application. Some aim to improve understanding of the increasingly important problem of dental erosion and the associated tooth wear process. Others monitor the changing pattern of caries in different populations, explore improved methods of diagnosis or evaluate methods of prevention or treatment. The broad coverage of current research has given the journal an international reputation as an indispensable source for both basic scientists and clinicians engaged in understanding, investigating and preventing dental disease.