Characterization of giant endocrine cells in the fundic stomach of African catfish (Clarias gariepinus) demonstrated by histochemical, immunohistochemical and ultrastructure microscopy methods suggesting their role in immunity

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

BMC Veterinary Research Pub Date : 2024-09-14 DOI:10.1186/s12917-024-04237-y

Hanan H. Abd-El-Hafeez, Sulaiman Mohammed Alnasser, Zyad M. Baker, Mohamed Aref, Mohamed A.M. Alsafy, Samir A.A. El-Gendy, Eman Zahran, Hams Mohamed M. A., Ali H. Alghamdi, Mahmoud Osman Khalifa, Basma M. Kamal, Fawzyah A. Alghamdi, Soha A. Soliman, Diaa Massoud

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引用次数: 0

Abstract

Endocrine cells in the fundic stomach of Clarias gariepinus were characterized in this work using transmission electron microscopy, immunohistochemistry, and histochemistry. Performic acid mixed with alcian blue pH2.5 and silver stain were among the histochemical stains used for endocrine cells. Endocrine cells can be found in the epithelium, lamina propria, submucosa, muscular layer, serosa, and the area between the stomach glands. Endocrine cells with one or more nuclei were found. Endocrine cells were studied using CD3, CD21, and CD68 in an immunohistochemistry analysis. The expression of the lymphocyte marker CD3 by endocrine cells is remarkable. In addition, they had a strong immunological response to CD21 and CD68, which are characteristics of phagocytic cells. Granules of varied sizes and electron densities are packed densely into the cytoplasm of the cells, as seen by transmission electron microscopy. We propose that endocrine cells play a crucial role in immune defense. The role of endocrine cells in the gut’s immune system is an area that needs further investigation.

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通过组织化学、免疫组织化学和超微结构显微镜方法观察非洲鲶鱼胃底巨型内分泌细胞的特征，提示其在免疫中的作用

本研究利用透射电子显微镜、免疫组织化学和组织化学方法对石斑鱼胃底的内分泌细胞进行了表征。内分泌细胞的组织化学染色方法包括过甲酸混合藻蓝 pH2.5 和银染色。内分泌细胞可见于上皮、固有层、黏膜下层、肌层、浆膜和胃腺体之间的区域。内分泌细胞有一个或多个细胞核。免疫组化分析中使用 CD3、CD21 和 CD68 对内分泌细胞进行了研究。内分泌细胞显著表达淋巴细胞标记 CD3。此外，它们对作为吞噬细胞特征的 CD21 和 CD68 有强烈的免疫反应。透射电子显微镜观察到，细胞的胞浆中密布着大小不一、电子密度不同的颗粒。我们认为，内分泌细胞在免疫防御中起着至关重要的作用。内分泌细胞在肠道免疫系统中的作用是一个需要进一步研究的领域。

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来源期刊

BMC Veterinary Research VETERINARY SCIENCES-

CiteScore

4.80

自引率

3.80%

发文量

420

审稿时长

3-6 weeks

期刊介绍： BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.