Effects of short-term oral letrozole on fresh semen parameters, endocrine balance, and prostate gland dimensions in domestic dogs

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Asghar Mogheiseh, Nooshin Derakhshandeh, Mohammad-Reza Divar, Saeed Nazifi, Iman Ahmadi
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Abstract

Aromatase inhibitors improve male fertility by modifying the hormonal control of spermatogenesis. The present study aimed to investigate the effects of oral administration of letrozole on testosterone and estradiol concentrations and their ratios in blood serum, seminal plasma, prostatic fluid, sperm quality in fresh semen, and prostate gland dimensions. Seven adult male intact mixed-breed dogs were selected. The animals received letrozole (72 µg/kg, PO) daily for four weeks. Blood samplings and semen collections were carried out on days 0 (control), 14 (treatment), 28 (treatment), and 42 (post-treatment). Our results showed that letrozole administration resulted in a 4.3 fold significant increase in serum, seminal plasma, and prostatic fluid testosterone levels after 14 days. This remained high until the end of the study. Serum and prostatic fluid estradiol levels did not change significantly over the study period. However, the seminal plasma estradiol level showed a significant increase on day 14. The estradiol: testosterone ratio was significantly reduced on day 14 in serum, seminal plasma, and prostatic fluid samples. Letrozole significantly improved the ejaculated spermatozoa viability and concentration after 28 days of oral administration. However, the sperm plasma membrane functional integrity and kinematic parameters were not significantly affected by the treatment. Transabdominal ultrasound examination revealed a significant increase in the height, width, and volume of the prostate gland after 28 days of treatment. According to the present research, oral administration of letrozole for 28 days affects local and systemic sex hormone balance leading to an improvement of the ejaculated canine spermatozoa viability and concentration concurrent with an increase in the prostate gland dimensions.
短期口服来曲唑对家犬新鲜精液参数、内分泌平衡和前列腺尺寸的影响
芳香化酶抑制剂通过改变精子发生的激素控制来提高男性生育能力。本研究旨在探讨口服来曲唑对血清、精浆、前列腺液中睾酮和雌二醇浓度及其比例、新鲜精液中精子质量和前列腺尺寸的影响。研究人员挑选了七只成年雄性混种犬。这些动物每天接受来曲唑(72 µg/kg,PO)治疗,为期四周。分别在第 0 天(对照组)、第 14 天(治疗组)、第 28 天(治疗组)和第 42 天(治疗后)进行血液采样和精液采集。我们的研究结果表明,服用来曲唑 14 天后,血清、精浆和前列腺液中的睾酮水平显著增加了 4.3 倍。直到研究结束,睾酮水平仍保持在较高水平。血清和前列腺液中的雌二醇水平在研究期间没有显著变化。不过,精浆中的雌二醇水平在第 14 天出现了明显升高。第 14 天,血清、精浆和前列腺液样本中的雌二醇:睾酮比率明显降低。口服来曲唑 28 天后,射出精子的活力和浓度明显提高。然而,精子质膜功能完整性和运动参数并未受到治疗的明显影响。经腹超声波检查显示,治疗 28 天后,前列腺的高度、宽度和体积明显增加。根据本研究,口服来曲唑 28 天会影响局部和全身的性激素平衡,从而提高射出犬精子的活力和浓度,同时增加前列腺的尺寸。
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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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