Characterization of doubled haploids obtained by in vitro androgenesis in African marigold (Tagetes erecta L.)

Abstract

Doubled haploids have the great potential to enhance both breeding efficiency and genetic research in African marigold (Tagetes erecta L.). In order to obtain basic information about doubled haploids in African marigold, in the present study, we investigated the morphological characters in doubled haploids generated through anther culture. Screening of anther derived regenerants revealed that out of the 72 plants, 6 plants (8.33%) were found to be haploids (2n = x = 12), 66 plants (91.66%) were found to be diploids (2n = 2x = 24) similar to its donor parent. Six doubled haploid lines hence homozygous material were obtained by in vitro anther culture from African marigold genotype Af/R/L-1 which were subsequently characterised for various qualitative and quantitative traits. In terms of results, a single doubled haploid line was not superior for every attribute, and distinct doubled haploid lines were superior for different qualities associated to growth and flowering. Different growth rates and genetic makeup could be the cause of this.