Fluorescence-detection size-exclusion chromatography specifically detects autoantibodies targeting the ganglionic acetylcholine receptor in patients with autoimmune autonomic ganglionopathy

IF 2.9 4区医学 Q3 IMMUNOLOGY

Journal of neuroimmunology Pub Date : 2024-09-08 DOI:10.1016/j.jneuroim.2024.578454

Leah Baxter , Steven Hopkins , Kevin C. O'Connor , Minh C. Pham , Richard J. Nowak , Nancy L. Monson , Kyle Blackburn , Ryan E. Hibbs , Steven Vernino , Colleen M. Noviello

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引用次数: 0

Abstract

Autoimmune autonomic ganglionopathy (AAG) is a rare disease wherein autoantibodies target the ganglionic acetylcholine receptor (gAChR). Current diagnosis in the United States depends upon clinical symptoms and positive autoantibody detection using a radioimmunoprecipitation assay (RIA). Here we offer a proof-of-principle study on an alternative method, fluorescence-detection size-exclusion-chromatography (FSEC). We show FSEC can detect autoantibodies against gAChR from patient sera but not healthy controls or samples from other autoimmune diseases. We compare FSEC to RIA and find good correlation. We discuss potential advantages of using FSEC as an alternative or as a first-step diagnostic prior to pursuing existing methodologies.

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荧光检测大小排阻色谱法特异性检测自身免疫性自主神经节病患者体内针对神经节乙酰胆碱受体的自身抗体

自身免疫性自主神经节病（AAG）是一种罕见疾病，其自身抗体靶向神经节乙酰胆碱受体（gAChR）。美国目前的诊断依据是临床症状和使用放射免疫沉淀法（RIA）检测到的阳性自身抗体。在此，我们对另一种方法--荧光检测尺寸排阻色谱法（FSEC）进行了原理验证研究。我们的研究表明，FSEC 能从患者血清中检测出针对 gAChR 的自身抗体，而不能从健康对照组或其他自身免疫性疾病的样本中检测出这种抗体。我们将 FSEC 与 RIA 进行了比较，发现两者具有良好的相关性。我们讨论了使用 FSEC 作为替代方法或在采用现有方法之前作为第一步诊断的潜在优势。

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来源期刊

Journal of neuroimmunology 医学-免疫学

CiteScore

6.10

自引率

3.00%

发文量

154

审稿时长

37 days

期刊介绍： The Journal of Neuroimmunology affords a forum for the publication of works applying immunologic methodology to the furtherance of the neurological sciences. Studies on all branches of the neurosciences, particularly fundamental and applied neurobiology, neurology, neuropathology, neurochemistry, neurovirology, neuroendocrinology, neuromuscular research, neuropharmacology and psychology, which involve either immunologic methodology (e.g. immunocytochemistry) or fundamental immunology (e.g. antibody and lymphocyte assays), are considered for publication.