Serial lift-out for in situ structural biology of multicellular specimens

IF 36.1 1区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Nature Methods Pub Date : 2024-09-13 DOI:10.1038/s41592-024-02317-3

Zhexin Wang, Tanmay A. M. Bharat

引用次数: 0

Abstract

The capability of high-resolution in situ imaging by electron cryo-tomography (cryo-ET) has now been expanded to large multicellular tissues by newly developed workflows involving lift-out and serial sectioning using focused ion beam milling under cryogenic conditions.

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用于多细胞标本原位结构生物学的串联移出技术

通过电子低温断层扫描（cryo-ET）进行高分辨率原位成像的能力现已扩展到大型多细胞组织，新开发的工作流程包括在低温条件下使用聚焦离子束铣削技术进行抬出和连续切片。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Methods 生物-生化研究方法

CiteScore

58.70

自引率

1.70%

发文量

326

审稿时长

1 months

期刊介绍： Nature Methods is a monthly journal that focuses on publishing innovative methods and substantial enhancements to fundamental life sciences research techniques. Geared towards a diverse, interdisciplinary readership of researchers in academia and industry engaged in laboratory work, the journal offers new tools for research and emphasizes the immediate practical significance of the featured work. It publishes primary research papers and reviews recent technical and methodological advancements, with a particular interest in primary methods papers relevant to the biological and biomedical sciences. This includes methods rooted in chemistry with practical applications for studying biological problems.

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