The Isolation of 5-Hydroxymethylfuran Metabolites from the Broth Extract of Fomitopsis meliae (Agaricomycetes)

IF 1.4 4区生物学 Q4 MYCOLOGY

International journal of medicinal mushrooms Pub Date : 2024-01-01 DOI:10.1615/intjmedmushrooms.2024055584

Siriwan Srisit, Chanyaporn Bunloed, Wichayaporn Soma, Jiraporn Panchompoo, Chiraphat Takpho, Ruchilak Rattarom, Aphidech Sangdee, Prapairat Seephonkai

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引用次数: 0

Abstract

This study aimed to identify antibacterial compounds from the broth extract of Fomitopsis meliae (MSUCC009). From small-scale fermentation, the broth extract of F. meliae showed antibacterial activity. Therefore, the fermentation of this fungal strain was scaled up and the broth extract was chemically investigated. Purification of the broth extract led to the isolation of two 5-hydroxymethylfuran metabolites, 5-hydroxymethyl-2- furoic acid methyl ester and 5-hydroxymethyl-2-furancarboxylic acid (HMFCA) together with a pyrimidine base, uracil. This is the first isolation report of 5-hydroxymethylfuran derivatives from the genus Fomitopsis. The structures of isolated compounds were elucidated based on nuclear magnetic resonance (NMR) and mass spectrometry (MS) spectroscopic methods, and comparison with previous reports. 5-Hydroxymethyl-2-furancarboxylic acid exhibited antibacterial activity against methicillin-susceptible Staphylococcus aureus with MIC and MBC values of > 0.25 mg/mL. 5-Hydroxymethyl-2-furoic acid methyl ester and 5-hydroxymethyl-2-furancarboxylic acid were also tested against A549 cancer cell lines. These two compounds were inactive in this cytotoxicity assay.

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从 Fomitopsis meliae（姬松茸）肉汤提取物中分离出 5-羟甲基呋喃代谢物

本研究旨在从 Fomitopsis meliae（MSUCC009）的肉汤提取物中鉴定抗菌化合物。通过小规模发酵，F. meliae 的肉汤提取物显示出抗菌活性。因此，扩大了该真菌菌株的发酵规模，并对肉汤提取物进行了化学研究。对肉汤提取物进行纯化后，分离出了两种 5-羟甲基呋喃代谢物：5-羟甲基-2-糠酸甲酯和 5-羟甲基-2-呋喃羧酸（HMFCA）以及一种嘧啶碱--尿嘧啶。这是首次从 Fomitopsis 属中分离出 5-羟甲基呋喃衍生物。根据核磁共振（NMR）和质谱（MS）光谱方法，并与以前的报道进行比较，阐明了分离化合物的结构。5-Hydroxymethyl-2-furancarboxylic acid 对甲氧西林敏感的金黄色葡萄球菌具有抗菌活性，其 MIC 和 MBC 值分别为 > 0.25 mg/mL。5- 羟甲基-2-糠酸甲酯和 5- 羟甲基-2-糠羧酸还针对 A549 癌细胞株进行了测试。这两种化合物在细胞毒性试验中没有活性。

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来源期刊

International journal of medicinal mushrooms 医学-药学

CiteScore

2.60

自引率

16.70%

发文量

审稿时长

6-12 weeks

期刊介绍： The rapid growth of interest in medicinal mushrooms research is matched by the large number of disparate groups that currently publish in a wide range of publications. The International Journal of Medicinal Mushrooms is the one source of information that will draw together all aspects of this exciting and expanding field - a source that will keep you up to date with the latest issues and practice. The International Journal of Medicinal Mushrooms published original research articles and critical reviews on a broad range of subjects pertaining to medicinal mushrooms, including systematics, nomenclature, taxonomy, morphology, medicinal value, biotechnology, and much more.