Fei-Feng Wang, Peng Qin, Meng-Ke Zhang, Ze-En Xiong, Andrew G. S. Cuthbertson, Sulaiman Ali Alharbi, Sajid Fiaz, Saleh Alfarraj, Mohammad Javed Ansari, Farrukh Azeem, Jian-Yu Meng, Wen Sang
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引用次数: 0
Abstract
Spodoptera litura (Lepidoptera: Noctuidae) is an important pest across a wide range of cropping industries. Due to increasing chemical resistance being displayed by this pest, the majority of conventional pesticides are now ineffective in controlling its populations. Therefore, it is essential that new target sites are identified to aid in the development of novel and efficient pest management strategies. To obtain a comprehensive understanding of transcription regulation in S. litura at various developmental stages, we created 33 libraries that comprised three replicates of nine development stages and two genders. The results of differentially expressed transcripts showed a wide difference within S. litura during its developmental process, and some genes were specifically expressed in certain developmental stages. To understand the function of gene expression at different developmental stages, all DEGs were mapped in the GO and KEGG databases. The results showed that the DEGs produced in the comparison between male pupae (MP) and sixth-instar larvae (L6) had the most abundant GO enrichment, followed by comparison of female pupae vs L6 and first-instar larvae (L1) vs eggs (E); L1 vs E had the most abundant enrichment with up-regulation in KEGG database, followed by the fifth instar larvae vs forth-instar larvae (L4); L4 vs third-instar larvae (L3) had the least abundant enrichment in the 10 comparative groups, followed by L3 vs second-instar larvae. This study analyzed the genetic variation within S. litura during its developmental process, to potentially identify an appropriate life stage at which control methods could be deployed to disrupt its development.
期刊介绍：
Gesunde Pflanzen publiziert praxisbezogene Beiträge zum Pflanzenschutz in Landwirtschaft, Forstwirtschaft, Gartenbau und öffentlichem Grün und seinen Bezügen zum Umwelt- und Verbraucherschutz sowie zu Rechtsfragen.
Das Themenspektrum reicht von der Bestimmung der Schadorganismen über Maßnahmen und Verfahren zur Minderung des Befallsrisikos bis hin zur Entwicklung und Anwendung nicht-chemischer und chemischer Bekämpfungsstrategien und -verfahren, aber auch zu Fragen der Auswirkungen des Pflanzenschutzes auf die Umwelt, die Sicherung der Ernährung sowie zu allgemeinen Fragen wie Nutzen und Risiken und zur Entwicklung neuer Technologien.
Jedes Heft enthält Originalbeiträge renommierter Wissenschaftler, aktuelle Informationen von Verbänden sowie aus der Industrie, Pressemitteilungen und Personalia.
Damit bietet die Zeitschrift vor allem Behörden und Anwendern im Agrarsektor und Verbraucherschutz fundierte Praxisunterstützung auf wissenschaftlichem Niveau.
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