Cryptic transmission and novel introduction of Dengue 1 and 2 genotypes in Colombia

IF 5.5 2区医学 Q1 VIROLOGY

Virus Evolution Pub Date : 2024-08-31 DOI:10.1093/ve/veae068

David Martínez, Marcela Gómez, Carolina Hernández, Sandra Campo-Palacio, Marina González-Robayo, Marcela Montilla, Norma Pavas-Escobar, Catalina Tovar-Acero, Lillys Geovo-Arias, Esilda Valencia-Urrutia, Nayade Córdoba-Renteria, Marlen Y Carrillo-Hernandez, Julian Ruiz-Saenz, Marlen Martinez-Gutierrez, Alberto Paniz-Mondolfi, Luz H Patiño, Marina Muñoz, Juan David Ramírez

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引用次数: 0

Abstract

Dengue fever remains as a public health challenge in Colombia, standing as the most prevalent infectious disease in the country. The cyclic nature of dengue epidemics, occurring approximately every three years, is intricately linked to meteorological events like El Niño Southern Oscillation (ENSO). Therefore, the Colombian system faces challenges in genomic surveillance. This study aimed to evaluate local dengue virus (DENV) transmission and genetic diversity in four Colombian departments with heterogeneous incidence patterns (Department is first level territorial units in Colombia). For this study, we processed 26t6 serum samples to identify DENV. Subsequently, we obtained 118 genome sequences by sequencing DENV genomes from serum samples of 134 patients infected with DENV-1 and DENV-2 serotypes. The predominant serotype was DENV-2 (108/143), with the Asian-American (AA) genotype (91/118) being the most prevalent one. Phylogenetic analysis revealed concurrent circulation of two lineages of both DENV-2 AA and DENV-1 V, suggesting ongoing genetic exchange with sequences from Venezuela and Cuba. The continuous migration of Venezuelan citizens into Colombia can contribute to this exchange, emphasizing the need for strengthened prevention measures in border areas. Notably, the Time to Most Recent Common Ancestor analysis identified cryptic transmission of DENV-2 AA since approximately 2015, leading to the recent epidemic. This challenges the notion that major outbreaks are solely triggered by recent virus introductions, emphasizing the importance of active genomic surveillance. The study also highlighted the contrasting selection pressures on DENV-1 V and DENV-2 AA, with the latter experiencing positive selection, possibly influencing its transmissibility. The presence of a cosmopolitan genotype in Colombia, previously reported in Brazil and Peru, raises concerns about transmission routes, emphasizing the necessity for thorough DENV evolution studies. Despite limitations, the study underscores genomic epidemiology’s crucial role in early detection and comprehension of DENV genotypes, recommending the use of advanced sequencing techniques as an early warning system to help prevent and control dengue outbreaks in Colombia and worldwide.

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哥伦比亚登革热 1 号和 2 号基因型的隐性传播和新型引入

登革热仍然是哥伦比亚的一项公共卫生挑战，是该国最流行的传染病。登革热流行的周期性（大约每三年发生一次）与厄尔尼诺南方涛动（ENSO）等气象事件密切相关。因此，哥伦比亚的基因组监测系统面临着挑战。本研究旨在评估登革热病毒（DENV）在哥伦比亚四个具有不同发病模式的省（省是哥伦比亚的一级领土单位）的传播情况和遗传多样性。在这项研究中，我们处理了 26t6 份血清样本以鉴定 DENV。随后，我们对134名感染DENV-1和DENV-2血清型的患者血清样本中的DENV基因组进行测序，获得了118个基因组序列。最主要的血清型是 DENV-2（108/143），其中亚裔美国人（AA）基因型（91/118）最为流行。系统发生学分析表明，DENV-2 AA 和 DENV-1 V 的两个系同时存在，这表明与委内瑞拉和古巴的序列正在进行遗传交流。委内瑞拉公民向哥伦比亚的持续迁移可能会促成这种交换，这强调了在边境地区加强预防措施的必要性。值得注意的是，"最近共同祖先时间 "分析发现，DENV-2 AA 的隐性传播大约始于 2015 年，导致了最近的疫情。这挑战了重大疫情仅由近期病毒引入引发的观点，强调了积极开展基因组监测的重要性。研究还强调了DENV-1 V和DENV-2 AA的选择压力对比，后者经历了正选择，可能影响了其传播性。哥伦比亚出现了一种世界性的基因型，而此前在巴西和秘鲁也有报道，这引起了人们对传播途径的担忧，强调了对 DENV 演化进行深入研究的必要性。尽管存在局限性，但这项研究强调了基因组流行病学在早期检测和理解 DENV 基因型方面的关键作用，建议使用先进的测序技术作为早期预警系统，帮助预防和控制登革热在哥伦比亚和全球的爆发。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Virus Evolution Immunology and Microbiology-Microbiology

CiteScore

10.50

自引率

5.70%

发文量

108

审稿时长

14 weeks

期刊介绍： Virus Evolution is a new Open Access journal focusing on the long-term evolution of viruses, viruses as a model system for studying evolutionary processes, viral molecular epidemiology and environmental virology. The aim of the journal is to provide a forum for original research papers, reviews, commentaries and a venue for in-depth discussion on the topics relevant to virus evolution.