Yu. F. Krupyanskii, V. V. Kovalenko, N. G. Loiko, E. V. Tereshkin, K. B. Tereshkina, A. N. Popov
{"title":"The Structure of DNA in Anabiotic and Mummified Escherichia coli Cells","authors":"Yu. F. Krupyanskii, V. V. Kovalenko, N. G. Loiko, E. V. Tereshkin, K. B. Tereshkina, A. N. Popov","doi":"10.1134/s1990793124700441","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The structural organization of DNA in stressed (with increased stress resistance), anabiotic, and mummified cells obtained by introducing 4-hexylresorcinol in different concentrations at different stages of cell culture growth is studied using the synchrotron radiation diffraction technique. The experimental studies allow us to conclude that 4-hexylresorcinol is the initiator of the transition of cells into an anabiotic and mummified state in the stationary stage of growth. In the prestationary stage, in the studied concentration range, 4-hexylresorcinol initiates the transition of cells into a mummified state but not into an anabiotic state, which indicates that DNA is unprepared for the crystallization process in these bacteria. The structure of DNA inside a cell in an anabiotic dormant state (the almost complete absence of metabolism) and dormant state (starvation stress) coincide (form nanocrystalline structures). The data indicate the universality of DNA condensation or the universality of DNA protection by the Dps protein in the dormant state, regardless of the type of stress. The mummified state (the complete absence of metabolism, irreversible to life) is very different in structure (has no order within the cell).</p>","PeriodicalId":768,"journal":{"name":"Russian Journal of Physical Chemistry B","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Russian Journal of Physical Chemistry B","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1134/s1990793124700441","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHYSICS, ATOMIC, MOLECULAR & CHEMICAL","Score":null,"Total":0}
引用次数: 0
Abstract
The structural organization of DNA in stressed (with increased stress resistance), anabiotic, and mummified cells obtained by introducing 4-hexylresorcinol in different concentrations at different stages of cell culture growth is studied using the synchrotron radiation diffraction technique. The experimental studies allow us to conclude that 4-hexylresorcinol is the initiator of the transition of cells into an anabiotic and mummified state in the stationary stage of growth. In the prestationary stage, in the studied concentration range, 4-hexylresorcinol initiates the transition of cells into a mummified state but not into an anabiotic state, which indicates that DNA is unprepared for the crystallization process in these bacteria. The structure of DNA inside a cell in an anabiotic dormant state (the almost complete absence of metabolism) and dormant state (starvation stress) coincide (form nanocrystalline structures). The data indicate the universality of DNA condensation or the universality of DNA protection by the Dps protein in the dormant state, regardless of the type of stress. The mummified state (the complete absence of metabolism, irreversible to life) is very different in structure (has no order within the cell).
摘要 利用同步辐射衍射技术研究了在细胞培养生长的不同阶段引入不同浓度的 4-己基间苯二酚所获得的受激细胞（抗应激能力增强）、厌氧细胞和木乃伊化细胞中 DNA 的结构组织。通过实验研究，我们得出结论：在细胞生长的静止阶段，4-己基间苯二酚是使细胞过渡到厌氧状态和木乃伊化状态的启动因子。在所研究的浓度范围内，4-己基间苯二酚能使细胞进入木乃伊化状态，但不能使细胞进入无代谢状态。处于无代谢休眠状态（几乎完全没有新陈代谢）和休眠状态（饥饿应激）的细胞内的 DNA 结构是一致的（形成纳米结晶结构）。这些数据表明，在休眠状态下，无论应激类型如何，DNA 的凝结或 DNA 受 Dps 蛋白保护具有普遍性。木乃伊状态（完全没有新陈代谢，对生命不可逆）的结构则截然不同（细胞内没有秩序）。
期刊介绍：
Russian Journal of Physical Chemistry B: Focus on Physics is a journal that publishes studies in the following areas: elementary physical and chemical processes; structure of chemical compounds, reactivity, effect of external field and environment on chemical transformations; molecular dynamics and molecular organization; dynamics and kinetics of photoand radiation-induced processes; mechanism of chemical reactions in gas and condensed phases and at interfaces; chain and thermal processes of ignition, combustion and detonation in gases, two-phase and condensed systems; shock waves; new physical methods of examining chemical reactions; and biological processes in chemical physics.
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