Determination of DEET, Icaridin, and IR3535 in insect repellents using excitation-emission matrix (EEM) fluorescence spectroscopy and multiway calibration

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal Pub Date : 2024-09-07 DOI:10.1016/j.microc.2024.111601

Leonardo Francisco Rafael Lemes, Frederico Luis Felipe Soares, Noemi Nagata

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引用次数: 0

Abstract

Due to rising temperatures in recent years, there has been an increase in outbreaks of arboviral diseases like Dengue, chikungunya, Zika, and yellow fever, which are primarily transmitted by two mosquitos. The outbreak has sparked significant concern among the population and prompted countries to heighten their precautions, specially by increasing the use of insects repellents. Regarding the regulatory demands of these products, this study develops a novel analytical method for the determination of DEET, Icaridin, and IR3535 in insect repellents combining excitation-emission matrix (EEM) fluorescence spectroscopy with higher-order multivariate calibration techniques. For this purpose, a dataset comprising 21 samples containing the studied active ingredients was used to construct Parallel Factor Analysis (PARAFAC) and Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) models. As a result, DEET, Icaridin, and IR3535 were quantified with a RMSEP lower than 1.84% for PARAFAC and below 11.30% for MCR-ALS. The EJCR test confirmed the accuracy of the proposed methods, which agreed with the high-performance liquid chromatography reference method at a 95% confidence level. These findings suggest that developing multiway calibration models using EEM proves to be accurate and cost-effective for quality control in repellent-based products.

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利用激发-发射矩阵 (EEM) 荧光光谱法和多路校准法测定驱虫剂中的 DEET、淫羊藿苷和 IR3535

由于近年来气温升高，登革热、基孔肯雅病、寨卡病和黄热病等主要由两种蚊子传播的虫媒病毒疾病爆发增多。疫情的爆发引发了民众的极大关注，促使各国加强预防措施，特别是增加驱虫剂的使用。针对这些产品的监管要求，本研究开发了一种新的分析方法，结合激发-发射矩阵（EEM）荧光光谱法和高阶多元校准技术，测定驱虫剂中的 DEET、淫羊藿苷和 IR3535。为此，我们使用了由 21 个含有所研究活性成分的样品组成的数据集来构建平行因子分析（PARAFAC）和多变量曲线分辨率-替代最小二乘法（MCR-ALS）模型。结果表明，DEET、淫羊藿苷和 IR3535 的 PARAFAC RMSEP 低于 1.84%，MCR-ALS RMSEP 低于 11.30%。EJCR 测试证实了拟议方法的准确性，在 95% 的置信水平上与高效液相色谱参考方法一致。这些研究结果表明，使用 EEM 建立多途径校准模型在驱避剂产品的质量控制方面具有准确性和成本效益。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microchemical Journal 化学-分析化学

CiteScore

8.70

自引率

8.30%

发文量

1131

审稿时长

1.9 months

期刊介绍： The Microchemical Journal is a peer reviewed journal devoted to all aspects and phases of analytical chemistry and chemical analysis. The Microchemical Journal publishes articles which are at the forefront of modern analytical chemistry and cover innovations in the techniques to the finest possible limits. This includes fundamental aspects, instrumentation, new developments, innovative and novel methods and applications including environmental and clinical field. Traditional classical analytical methods such as spectrophotometry and titrimetry as well as established instrumentation methods such as flame and graphite furnace atomic absorption spectrometry, gas chromatography, and modified glassy or carbon electrode electrochemical methods will be considered, provided they show significant improvements and novelty compared to the established methods.

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