An ATP-fluorescence dual-mode biosensing platform for rapid broad-spectrum enrichment and detection of bacteria based on PEI-functionalized magnetic UiO-66-NH2

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal Pub Date : 2024-09-03 DOI:10.1016/j.microc.2024.111560

Qijun He, Bin Du, Wei Hu, Lingxiao Liu, Bing Liu, Xihui Mu, Zhaoyang Tong, Rabigul Tursun, Jianjie Xu

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Abstract

Bacteria-induced food pollution poses a serious threat to human health. To detect early and sensitive traces of bacteria in food, a poly (ethyleneimine) (PEI)-modified magnetic organic framework material (FeO@UiO-66-NH@PEI) was prepared for broad-spectrum enrichment and dual-mode detection of typical gram-positive bacteria (Bacillus subtilis, B. subtilis) and gram-negative bacteria (Escherichia coli, E. coli) using Schiff’s base reaction. Due to the positive charge caused by the abundance of amino groups, FeO@UiO-66-NH@PEI exhibited rapid enrichment of E. coli and B. subtilis within 12 min through the electrostatic interaction with bacteria. The biosensor based on FeO@UiO-66-NH@PEI achieved sensitive detection of E. coli and B. subtilis using the adenosine triphosphate (ATP) bioluminescence technology within the detection range of 10-10, and the detection limits were 44 and 57 CFU/mL, respectively under optimal experimental conditions. Moreover, a fluorescence platform was constructed by FeO@UiO-66-NH@PEI to achieve more diversified detection and reliable detection results for E. coli and B. subtilis. Within the detection range of 10-10, FeO@UiO-66-NH@PEI exhibited satisfactory linearity with detection limits of 6 CFU/mL and 14 CFU/mL. This biosensor using ATP bioluminescence and fluorescence technology showed remarkable recoveries (85.3–108.5 %) for E. coli and B. subtilis in biscuit matrices, indicating a bright prospect of the dual-mode platform for bacteria detection in food.

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基于 PEI 功能化磁性 UiO-66-NH2 的 ATP 荧光双模式生物传感平台，用于快速广谱富集和检测细菌

细菌引起的食品污染严重威胁人类健康。为了早期灵敏地检测食品中的细菌痕量，本研究制备了一种聚（乙烯亚胺）（PEI）修饰的磁性有机框架材料（FeO@UiO-66-NH@PEI），利用席夫碱反应对典型的革兰氏阳性菌（枯草杆菌，B. subtilis）和革兰氏阴性菌（大肠杆菌，E. coli）进行广谱富集和双模式检测。由于氨基酸基团的丰富性所产生的正电荷，FeO@UiO-66-NH@PEI 通过与细菌的静电作用在 12 分钟内迅速富集了大肠杆菌和枯草杆菌。基于 FeO@UiO-66-NH@PEI 的生物传感器利用三磷酸腺苷（ATP）生物发光技术实现了对大肠杆菌和枯草杆菌的灵敏检测，检测范围为 10-10，在最佳实验条件下检测限分别为 44 和 57 CFU/mL。此外，FeO@UiO-66-NH@PEI 构建的荧光平台实现了对大肠杆菌和枯草杆菌更多样化的检测和更可靠的检测结果。在 10-10 的检测范围内，FeO@UiO-66-NH@PEI 表现出令人满意的线性，检测限分别为 6 CFU/mL 和 14 CFU/mL。这种采用 ATP 生物发光和荧光技术的生物传感器对饼干基质中的大肠杆菌和枯草杆菌的回收率非常高（85.3-108.5%），表明这种双模式平台在食品中细菌检测方面具有广阔的前景。

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来源期刊

Microchemical Journal 化学-分析化学

CiteScore

8.70

自引率

8.30%

发文量

1131

审稿时长

1.9 months

期刊介绍： The Microchemical Journal is a peer reviewed journal devoted to all aspects and phases of analytical chemistry and chemical analysis. The Microchemical Journal publishes articles which are at the forefront of modern analytical chemistry and cover innovations in the techniques to the finest possible limits. This includes fundamental aspects, instrumentation, new developments, innovative and novel methods and applications including environmental and clinical field. Traditional classical analytical methods such as spectrophotometry and titrimetry as well as established instrumentation methods such as flame and graphite furnace atomic absorption spectrometry, gas chromatography, and modified glassy or carbon electrode electrochemical methods will be considered, provided they show significant improvements and novelty compared to the established methods.