Detection of Clubroot Disease Resistance in Brassica juncea Germplasm at the Seedling Stage

Agronomy Pub Date : 2024-09-06 DOI:10.3390/agronomy14092042

Wenlong Yang, Jiangping Song, Xiaohui Zhang, Chu Xu, Jiaqi Han, Zhijie Li, Yang Wang, Huixia Jia, Haiping Wang

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Abstract

Infection by the mustard clubroot disease pathogen Plasmodiophora brassicae has a significant negative impact on the quality and yield of Chinese mustard (Brassica juncea). At present, screening resistant resources for breeding programs is the most economical and effective method available to control this disease. In this study, we isolated P. brassicae physiological race 4 from Chinese cabbage and examined 483 mustard germplasm resources (193 leaf mustard, 96 stem mustard, and 194 root mustard) from China and abroad to identify resistance to clubroot disease at the seedling stage through irrigation inoculation with the isolated pathogen. The results showed that there were no immune varieties among the tested mustard germplasm, but that there were differences in resistance to clubroot disease among the three mustard types. More than 90% of leaf and stem mustard resources were susceptible to clubroot disease, whereas 38.66% of root mustard resources showed resistance. In total, we detected 4 highly resistant, 9 resistant, and 83 moderately resistant varieties, of which 4 highly resistant, 8 resistant, and 63 moderately resistant varieties were root mustard resources, whereas only 1 resistant and 5 moderately resistant varieties were stem mustard resources, and 15 moderately resistant varieties were leaf mustard resources. In addition, we used seven molecular markers for clubroot disease resistance in Chinese cabbage to detect stem and root mustard resources. The results showed that the marker CRk was detected in 97.87% of stem mustard and 92.49% of root mustard resources. Six markers (Crr1, Crr2, Crr3, CRa, CRb, and CRc) were detected in 18.09%, 7.45%, 2.13%, 6.38%, 12.77%, and 12.77% of stem mustard germplasms, and four markers (Crr1, Crr2, Crr3, and CRc) were detected in 8.09%, 8.67%, 10.40%, and 8.67% of root mustard germplasms, respectively, suggesting that these markers are not suitable for detecting mustard germplasm resistance to clubroot disease. This study provides a technical reference and material support for the breeding of mustard varieties resistant to clubroot disease.

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苗期检测甘蓝种质的抗球虫病能力

芥菜棒根病病原菌 Plasmodiophora brassicae 的感染对芥菜（Brassica juncea）的质量和产量有很大的负面影响。目前，为育种计划筛选抗病资源是控制该病害最经济、最有效的方法。在本研究中，我们从大白菜中分离出了P. brassicae生理竞赛4，并考察了国内外483份芥菜种质资源（193份叶用芥菜、96份茎用芥菜和194份根用芥菜），通过灌溉接种分离出的病原菌，鉴定其对苗期棒根病害的抗性。结果表明，受试芥菜种质资源中不存在免疫品种，但三种芥菜类型对棒根病的抗性存在差异。超过 90% 的芥菜叶片和茎资源易感球虫病，而 38.66% 的芥菜根资源表现出抗性。我们总共发现了 4 个高抗、9 个抗性和 83 个中抗品种，其中根芥资源有 4 个高抗、8 个抗性和 63 个中抗品种，而茎芥资源只有 1 个抗性和 5 个中抗品种，叶芥资源有 15 个中抗品种。此外，我们还利用大白菜抗球虫病的 7 个分子标记来检测茎芥和根芥资源。结果表明，在 97.87% 的茎用芥菜和 92.49% 的根用芥菜资源中检测到了标记 CRk。6个标记（Crr1、Crr2、Crr3、CRa、CRb和CRc）分别在18.09%、7.45%、2.13%、6.38%、12.77%和12.77%的茎芥种质中检测到，4个标记（Crr1、Crr2、Crr3和CRc）分别在8.09%、8.67%、10.40% 和 8.67%，表明这些标记不适合用于检测芥菜种质对棍棒病的抗性。该研究为培育抗球根病芥菜品种提供了技术参考和材料支持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Agronomy

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