Triple-color FISH chromosome constitution and genome size analyses confirmed the ploidy of newly developed ten Hibiscus Sp. Cultivars with different breeding history

IF 2.4 3区 农林科学 Q1 Agricultural and Biological Sciences
Saraswathi Umavathi, Byung Yong Kang, Hae-Yun Kwon, Hanna Shin, Hyun Hee Kim
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Abstract

Hibiscus syriacus L. (Rose of Sharon), is the national flower of Korea. H. syriacus has both ornamental and medicinal value, and many cultivars have been exploited for its development. Cytogenomic information is crucial for elucidating chromosomal composition, ploidy, and specific sequence distribution. In this study, chromosome constitution, genome size, and ploidy were analyzed in ten Hibiscus sp. cultivars with different breeding histories using triple-color fluorescence in situ hybridization (FISH) and flow cytometry. Prelabeled oligonucleotide probes (PLOPs) of three universal tandem repeats (5S rDNA, 45S rDNA, and telomeric DNA) were used for FISH. The chromosome length ranged from 1.20 to 4.84 μm, and the chromosome number varied from 2n = 84 to 2n = 172 with the basic chromosome number x = 21. Based on chromosome number, FISH signals, and genome size, three cultivars were identified as euploids of tetraploid and hexaploid euploids, and the remaining seven cultivars as aneuploids of tetraploid, hexaploid, and octoploid euploids. The number of 5S rDNA signals was two, three, and four for tetraploid, hexaploid, and octoploid, respectively. Whereas 45S rDNA signals varied from two to five pairs among the cultivars. Genome size (2C) ranged from 4.35 to 4.80 pg in tetraploids, 6.35 to 6.78 pg in hexaploids, and 8.20 to 8.94 pg in octoploids, which corresponded to the ploidy identification using FISH chromosome composition. FISH using PLOPs demonstrated that the nuclear DNA content increased proportionally with the ploidy level, indicating that the chromosome composition and genome size were consistent. The findings will be useful for future genomic research on Hibiscus species breeding and development strategies.

Abstract Image

三色 FISH 染色体组成和基因组大小分析证实了新培育的 10 个芙蓉品种的倍性。不同育种历史的栽培品种
Hibiscus syriacus L.(沙仑玫瑰)是韩国的国花。H. syriacus 具有观赏和药用价值,许多栽培品种已被开发利用。细胞基因组信息对阐明染色体组成、倍性和特异序列分布至关重要。本研究利用三色荧光原位杂交(FISH)和流式细胞仪分析了十个具有不同育种历史的芙蓉品种的染色体组成、基因组大小和倍性。FISH 采用了三种通用串联重复序列(5S rDNA、45S rDNA 和端粒 DNA)的预标记寡核苷酸探针(PLOPs)。染色体长度从 1.20 μm 到 4.84 μm,染色体数从 2n = 84 到 2n = 172 不等,基本染色体数 x = 21。根据染色体数目、FISH 信号和基因组大小,3 个栽培品种被鉴定为四倍体和六倍体的优倍体,其余 7 个栽培品种被鉴定为四倍体、六倍体和八倍体的非优倍体。四倍体、六倍体和八倍体的 5S rDNA 信号数量分别为 2 个、3 个和 4 个。不同栽培品种的 45S rDNA 信号从 2 对到 5 对不等。四倍体的基因组大小(2C)在 4.35 至 4.80 pg 之间,六倍体在 6.35 至 6.78 pg 之间,八倍体在 8.20 至 8.94 pg 之间,这与利用 FISH 染色体组成进行的倍性鉴定相符。利用 PLOPs 进行的 FISH 显示,核 DNA 含量随着倍性水平的增加而成正比增加,这表明染色体组成和基因组大小是一致的。这些研究结果将有助于未来木槿物种育种和发展战略的基因组研究。
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来源期刊
Horticulture Environment and Biotechnology
Horticulture Environment and Biotechnology Agricultural and Biological Sciences-Horticulture
CiteScore
4.30
自引率
4.20%
发文量
0
审稿时长
6 months
期刊介绍: Horticulture, Environment, and Biotechnology (HEB) is the official journal of the Korean Society for Horticultural Science, was launched in 1965 as the "Journal of Korean Society for Horticultural Science". HEB is an international journal, published in English, bimonthly on the last day of even number months, and indexed in Biosys Preview, SCIE, and CABI. The journal is devoted for the publication of original research papers and review articles related to vegetables, fruits, ornamental and herbal plants, and covers all aspects of physiology, molecular biology, biotechnology, protected cultivation, postharvest technology, and research in plants related to environment.
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