Orsolya K. Zorkóczy, Adrienn Gyurcsó, László Ózsvári, Pál Lehotzky, Raveendranathanpillai Sanil, Petra Zenke
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引用次数: 0
Abstract
Determining sex in Cervidae species is crucial for wildlife management, conservation, breeding programs, disease surveillance, and forensic investigations. While existing genetic sexing methods have proven effective, they often involve time-consuming and resource-intensive processes. In response to the growing need for a more efficient and cost-effective technique, this study aimed to develop a novel loop-mediated isothermal amplification (LAMP) method for sex detection in various deer species. We designed LAMP primers specific to Cervidae based on sex chromosome-located genes, AmelogeninX, and SRY. Analytical specificity was confirmed using 108 samples from red deer, roe deer, and fallow deer, and species specificity was also tested in other deer and mammal species. Sex determination results were fast (40 min) and accurate (100% reliability). This technique has the advantage of allowing researchers to identify the sex of different deer species using the same method. Using the isothermal amplification properties of LAMP, this new method provides a simple, quick, and accurate tool to help researchers, wildlife managers, and forensic investigators with important sex-related questions in cervid populations.
期刊介绍:
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.