Development of a loop-mediated isothermal amplification technique for sex detection in Cervidae species

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Orsolya K. Zorkóczy, Adrienn Gyurcsó, László Ózsvári, Pál Lehotzky, Raveendranathanpillai Sanil, Petra Zenke
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引用次数: 0

Abstract

Determining sex in Cervidae species is crucial for wildlife management, conservation, breeding programs, disease surveillance, and forensic investigations. While existing genetic sexing methods have proven effective, they often involve time-consuming and resource-intensive processes. In response to the growing need for a more efficient and cost-effective technique, this study aimed to develop a novel loop-mediated isothermal amplification (LAMP) method for sex detection in various deer species. We designed LAMP primers specific to Cervidae based on sex chromosome-located genes, AmelogeninX, and SRY. Analytical specificity was confirmed using 108 samples from red deer, roe deer, and fallow deer, and species specificity was also tested in other deer and mammal species. Sex determination results were fast (40 min) and accurate (100% reliability). This technique has the advantage of allowing researchers to identify the sex of different deer species using the same method. Using the isothermal amplification properties of LAMP, this new method provides a simple, quick, and accurate tool to help researchers, wildlife managers, and forensic investigators with important sex-related questions in cervid populations.

Abstract Image

开发用于鹿科物种性别检测的环介导等温扩增技术
确定鹿科物种的性别对于野生动物管理、保护、繁殖计划、疾病监测和法医调查至关重要。虽然现有的基因性别鉴定方法已被证明是有效的,但这些方法往往需要耗费大量时间和资源。为了满足对更高效、更具成本效益的技术日益增长的需求,本研究旨在开发一种新型环介导等温扩增(LAMP)方法,用于各种鹿的性别检测。我们根据性染色体定位基因 AmelogeninX 和 SRY 设计了针对鹿科动物的 LAMP 引物。分析特异性是通过 108 份来自赤鹿、狍子和秋鹿的样本确认的,物种特异性也在其他鹿类和哺乳动物物种中进行了测试。性别测定结果快速(40 分钟)、准确(100% 可靠)。该技术的优点是研究人员可以用同一种方法鉴定不同鹿类的性别。利用 LAMP 的等温扩增特性,这种新方法提供了一种简单、快速和准确的工具,可帮助研究人员、野生动物管理人员和法医调查人员解决与鹿类种群性别相关的重要问题。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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