Enzymatic Decolorization of Dye Wastewater and its Effect on Respiration of Activated Sludge

IF 3.8 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Water, Air, & Soil Pollution Pub Date : 2024-09-04 DOI:10.1007/s11270-024-07478-7

Jiangmian Wei, Yanjun Chen, Mingxin Zhu, Jiayang Liu, Jun Wang

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Abstract

This study investigated the decolorization and detoxification of a dye originating from a feather-dyeing house using the laccase enzyme. Efficient decolorization was achieved under a wide range of environmental conditions, including pH, temperature, enzyme loading, and dye concentration. The decolorization rate reached up to 98% within 10 min when the mediator acetosyringone was present. Decolorization was attributed to the degradation of the dye by laccase, as analyzed using liquid chromatography-mass spectrometry (LC–MS). At least three dominant intermediates (m/z 167.993, 194.475, 118.040) were identified, deriving from the original dye (m/z 521.044). Compared to the non-decolorized dye solution, the decolorized dye ones at various concentrations exhibited lower toxicity towards aerobic activated sludge (AS). This was evident from the significantly higher respiratory intensity of AS, with an O₂ uptake of 35 mg for the decolorized solution versus 11 mg for the non-decolorized solution after 12 h at a 100 mg/L dye concentration. The results suggest that laccase-catalyzed decolorization could serve as an effective pretreatment method for traditional activated sludge (AS)-based processes, thereby enhancing the overall treatment performance of dye wastewater.

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染料废水的酶促脱色及其对活性污泥呼吸作用的影响

本研究利用漆酶对一种来自羽毛染色屋的染料进行脱色和解毒。在 pH 值、温度、酶载量和染料浓度等多种环境条件下，均实现了高效脱色。当媒介物乙酰丁香酮存在时，脱色率在 10 分钟内达到 98%。通过液相色谱-质谱（LC-MS）分析，脱色是由于漆酶降解了染料。从原始染料（m/z 521.044）衍生出至少三种主要中间体（m/z 167.993、194.475、118.040）。与未脱色的染料溶液相比，不同浓度的脱色染料对好氧活性污泥（AS）的毒性较低。在 100 毫克/升染料浓度下，12 小时后，脱色溶液的 O₂ 吸收量为 35 毫克，而非脱色溶液为 11 毫克。结果表明，漆酶催化脱色可作为传统活性污泥法的一种有效预处理方法，从而提高染料废水的整体处理性能。

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来源期刊

Water, Air, & Soil Pollution 环境科学-环境科学

CiteScore

4.50

自引率

6.90%

发文量

448

审稿时长

2.6 months

期刊介绍： Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments. Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation. Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.