Potentilla fulgens Wall. Ex sims. Upregulates insulin receptor substrate 1 and Akt in alloxan-induced diabetic mice

Clinical Phytoscience Pub Date : 2024-09-12 DOI:10.1186/s40816-024-00382-x

Shelareen Ediemi Sunn, Careen Liza Pakyntein, Daiahun Thabah, Cynthia Erica Kharshiing, Sagnik Banerjee, Anita Kumari Rai, Atanu Bhattacharjee, Donkupar Syiem

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Abstract

Potentilla fulgens Wall. ex Sims. is a medicinal plant used by the locals of Meghalaya. However, its mechanism of action has not been well elucidated. Hence, this study investigated the effect of P. fulgens on IRS1 and Akt. The interaction of the various polyphenols present in P. fulgens with the IR tyrosine kinase and IRS1 PTB domain was studied using auto dock. Changes in expression of antioxidant enzymes, IRS-1, Akt and behavior of normal, diabetic, and diabetic mice treated mice were assessed after 14 days of treatment. Morphological changes in the liver tissue were determined by Transmission Electron Microscopy. The effect of P. fulgens on blood glucose was time and dose dependent. Treatment with P. fulgens, Cat, E, CE, CEP and metformin improved the activity of catalase, glutathione peroxidase, glycogen, IRS-1 and Akt. The Forced Swimming test showed an altered behavior in diabetic mice. The altered mobility was reverted back to near normal on treatment with P.fulgens, Cat, E, CE, CEP and metformin. The morphological aberrations seen in diabetic animals considerably improved in the treated diabetic group. P. fulgens and its phytochemicals-catechin and epicatechin are potent sources of antidiabetic drugs, possibly mediating their effects through upregulation of insulin IRS-1 and Akt.

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Potentilla fulgens Wall.Ex sims.上调阿脲诱导的糖尿病小鼠的胰岛素受体底物 1 和 Akt

Potentilla fulgens Wall.然而，其作用机制尚未得到很好的阐明。因此，本研究调查了白花蛇舌草对 IRS1 和 Akt 的影响。研究人员使用自动对接法研究了富尔格仙人掌中的各种多酚与 IR 酪氨酸激酶和 IRS1 PTB 结构域之间的相互作用。治疗 14 天后，对正常小鼠、糖尿病小鼠和糖尿病小鼠的抗氧化酶、IRS-1、Akt 的表达和行为变化进行了评估。透射电子显微镜测定了肝组织的形态变化。白头翁对血糖的影响与时间和剂量有关。使用白头翁、Cat、E、CE、CEP和二甲双胍治疗可提高过氧化氢酶、谷胱甘肽过氧化物酶、糖原、IRS-1和Akt的活性。强迫游泳试验表明，糖尿病小鼠的行为发生了改变。在使用白头翁、Cat、E、CE、CEP 和二甲双胍治疗后，活动能力的改变恢复到接近正常水平。糖尿病动物的形态畸变在接受治疗的糖尿病组中得到明显改善。红景天及其植物化学物质儿茶素和表儿茶素是抗糖尿病药物的有效来源，可能通过上调胰岛素 IRS-1 和 Akt 发挥作用。

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来源期刊

Clinical Phytoscience

自引率

0.00%

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审稿时长

13 weeks

期刊介绍： Clinical Phytoscience is an international, peer-reviewed, interdisciplinary, and open access journal publishing high quality research articles on clinical evidence and use of medicinal plants in the development of efficient and well tolerated phytotherapy. Clinical Phytoscience focuses on phytotherapy, looking at proof of concept, efficacy and safety, to be established “at eye level” compared to pharmacotherapy. The emphasis lies on application oriented topics (efficacy and safety of phytotherapy in a specific indication, including its need and acceptance by the patient). The scientific results published in the journal should contribute to the recovery and maintenance of human health by phytotherapy. Clinical Phytoscience will publish high-quality evidence-based clinical studies and relevant pharmacological studies. Key areas of interest are: -Upper and lower airways, ENT and pneumology -Gynecology -Urology -Nephrology Pediatrics -Intestinal tract -Hepatology -Diabetes/metabolic Syndrome -Immunology and microbiology -Hygiene -Analytics