A multifunctional natural treasure based on a “one stone, many birds” strategy for designing health-promoting applications: Tordylium apulum

IF 4.8 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
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引用次数: 0

Abstract

Wild plants provide important bioactive compounds, and their analysis relies heavily on selecting the right extraction techniques and solvents. This study was conducted to determine the phenolic content and biopharmaceutical potential of four different extracts (ethyl acetate, ethanol, 70% ethanol, and water) from the aerial parts of wild plant Tordylium apulum L. The biochemical profile of the extract was screened using high performance liquid chromatography -mass spectrometry (HPLC-MS) analysis. The total phenolic and flavonoid content was examined using the Folin-Ciocalteu assay and the aluminium trichloride assay, respectively. The antioxidant activity was evaluated through several tests, including 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), cupric reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power (FRAP), phosphomolybdenum (PBD), and metal chelating activity (MCA). Five types of enzyme inhibition activity were tested against acetylcholinesterase (AChE), butrylcholinesterase (BChE), tyrosinase, α-amylase, and α-glucosidase. Additionally, For the first time, the inhibitory activity of T. apulum extract against human carbonic anhydrase isoenzymes I and II (hCA-I and hCA-II) was evaluated. Fifty-five compounds for negative ionization mode, and twenty-eight compounds for positive ionization mode were recorded in HPLC-MS analysis and they were polyphenolic, flavonoids, carbohydrates, sugar alcohol and amino acids. These results indicate that different solvents extract varying levels of antioxidants from T. apulum, with ethanol and water extracts generally exhibiting superior antioxidant activities. The ethanol extract of T. apulum exhibited the maximum contents of total phenolics measuring 33.71 mg gallic acid equivalent (GAE)/g. The ethanol extract exhibited the highest inhibition of AChE with 2.28 mg galanthamine equivalent (GALAE)/g. The ethyl acetate and ethanol extracts also showed the highest hCA-I and hCA-II inhibition potential, respectively. The ethanol-water and water extracts acted on the biofilm of E. coli (49.93% and 45.22%, respectively), and the biofilm of P. aeruginosa (50.68% and 44.46%, respectively). The extracts were tested on different cell lines for cytotoxic potentials and in particular the water extract induced the apoptotic pathways in cervical cancer (HELA) cell lines. In conclusion, T. apulum exhibit multidirectional biological properties and it could be considered as a versatile agent for the development of health-promoting applications.

基于 "一石多鸟 "战略的多功能天然宝藏,用于设计促进健康的应用:蕨类植物
野生植物可提供重要的生物活性化合物,对它们的分析在很大程度上依赖于选择正确的提取技术和溶剂。本研究从野生植物 Tordylium apulum L 的气生部分提取了四种不同的提取物(乙酸乙酯、乙醇、70% 乙醇和水),以确定其酚含量和生物制药潜力。采用 Folin-Ciocalteu 法和三氯化铝法分别检测了总酚和类黄酮的含量。抗氧化活性通过几种试验进行了评估,包括 2,2-二苯基-1-苦基肼(DPPH)、2,2′-偶氮-双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)、铜还原抗氧化能力(CUPRAC)、铁还原抗氧化能力(FRAP)、磷钼(PBD)和金属螯合活性(MCA)。测试了乙酰胆碱酯酶(AChE)、丁酰胆碱酯酶(BChE)、酪氨酸酶、α-淀粉酶和α-葡萄糖苷酶五种酶的抑制活性。此外,还首次评估了 T. apulum 提取物对人碳酸酐酶同工酶 I 和 II(hCA-I 和 hCA-II)的抑制活性。在 HPLC-MS 分析中记录到 55 种负离子模式化合物和 28 种正离子模式化合物,它们分别是多酚、黄酮类、碳水化合物、糖醇和氨基酸。这些结果表明,不同溶剂可从钟乳石中提取不同水平的抗氧化剂,其中乙醇和水提取物通常具有较高的抗氧化活性。T. apulum 的乙醇提取物中总酚类含量最高,达到 33.71 毫克没食子酸当量(GAE)/克。乙醇提取物对 AChE 的抑制率最高,为 2.28 毫克加兰他敏当量(GALAE)/克。乙酸乙酯和乙醇提取物对 hCA-I 和 hCA-II 的抑制潜力也分别最高。乙醇-水提取物和水提取物对大肠杆菌生物膜的抑制率分别为 49.93% 和 45.22%,对铜绿假单胞菌生物膜的抑制率分别为 50.68% 和 44.46%。对不同细胞系的提取物进行了细胞毒性潜力测试,特别是水提取物诱导了宫颈癌(HELA)细胞系的凋亡途径。总之,T. apulum 具有多向生物学特性,可被视为一种多功能制剂,用于开发促进健康的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Food Bioscience
Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
6.40
自引率
5.80%
发文量
671
审稿时长
27 days
期刊介绍: Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.
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