Fabrication of nanomolecular platform based immunosensor for non-invasive electrochemical detection of oral cancer: An in vitro study

IF 4.1 Q1 CHEMISTRY, ANALYTICAL

Talanta Open Pub Date : 2024-09-01 DOI:10.1016/j.talo.2024.100352

Meenakshi Choudhary , Vinay Kumar , Ashik Babu Parambath , Shruti Caplash , Birendra Kumar Yadav , Satbir Kaur , Sapna Ratan Shah , Kavita Arora

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Abstract

Silver nanoparticles (AgNPs)-Chitosan (Chi) functionalized cysteine (cys)-SAM (self-assembled monolayer) based electrochemical, label free, ultrasensitive, non-invasive immunosensing nanomolecular platform has been fabricated for the detection of novel OC biomarker S-100. This has been achieved through bio-functionalization of AgNPs-Chi/cys/mini gold slides by covalent immobilization of anti-S100 antibodies. Cyclic voltammetric (CV) studies establish successful fabrication of anti-S100/AgNPs-Chi/Au nanomolecular platform. Differential pulse voltammetric (DPV) measurements reveal that anti-S100/Chi-AgNPs/Au is able to sense 50 fg mL^-1 – 500 ng mL^-1 of S100 in buffer and in spiked saliva samples within 120 s interaction time having improved favorable shelf life and specificity. The fabricated platform is very selective and able to detect S100 in OC patient/s saliva samples warrants realization of simple non-invasive electrochemical immunosensor for in situ or on-site applications (especially for remote areas) for early diagnosis of OC while offering bio-compatibility at low cost.

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基于纳米分子平台的免疫传感器的制作，用于口腔癌的无创电化学检测：体外研究

为检测新型 OC 生物标志物 S-100，我们制作了基于银纳米粒子（AgNPs）-壳聚糖（Chi）-半胱氨酸（cys）-SAM（自组装单层）的电化学、无标记、超灵敏、非侵入性免疫传感纳米分子平台。这是通过共价固定抗 S100 抗体对 AgNPs-Chi/cys/mini 金片进行生物功能化实现的。循环伏安法（CV）研究证实了抗 S100/AgNPs-Chi/Au 纳米分子平台的成功制备。差分脉冲伏安法（DPV）测量结果表明，抗 S100/Chi-AgNPs/Au 能够在 120 秒的相互作用时间内感知缓冲液和加标唾液样品中 50 fg mL-1 - 500 ng mL-1 的 S100，并具有更好的保存期限和特异性。所制作的平台具有很高的选择性，能够检测 OC 患者/唾液样本中的 S100，因此可以实现简单的无创电化学免疫传感器，用于原位或现场应用（尤其是偏远地区），对 OC 进行早期诊断，同时具有低成本的生物兼容性。

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