Immune response for acute Aeromonas hydrophila infection in two distinct color morphs of northern snakehead, Channa argus

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Chong Wang, Qingsong Shu, Nanyang Zeng, Shaolin Xie, Jixing Zou, Huijuan Tang, Aiguo Zhou
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引用次数: 0

Abstract

To compare and analyze the differences in immunological response between the two color morphs of Channa argus, a fish cohort was divided into four groups: black C argus + PBS (B-PBS), black C argus + Aeromonas hydrophila (B-Ah), white C. argus + PBS (W-PBS), and white C. argus + A hydrophila (W-Ah). The B-PBS and W-PBS groups received 100 μL PBS, while the B-Ah and W-Ah groups received 3.6 × 105 CFU/mL A. hydrophila in the same volume. The death rate in each group was noted, changes in plasma biochemical indicators and the expression of liver immune-related genes were examined, and transcriptome techniques were used to compare the differences between the two colors of C. argus following stress. No mortality occurred in the B-PBS and W-PBS groups. Mortality in the W-Ah and B-Ah groups showed an upward and then downward trend after A. hydrophila injection. The highest mortality occurred within 24 h and was higher in the W-Ah group than in the B-Ah group. MDA levels in the B-Ah and W-Ah groups increased and then decreased, while SOD and T-AOC showed the reverse tendency. The W-Ah and W-PBS groups differed significantly in MDA at 3, 12, and 24 h, SOD from 6 to 96 h, and T-AOC between 6 and 48 h. Plasma MDA and T-AOC levels at 12 h and SOD levels at 24 and 48 h differed significantly between the B-PBS and B-Ah groups. In both the W-Ah and B-Ah groups, the expression levels of IL-1β and IL-8 in the liver showed a temporal pattern with an initial increase followed by a decrease, reaching peak levels after 24 h, while IL-10 showed the reverse pattern. Transcriptome analysis of the liver revealed significant differences between the two C. argus colors. Differential genes in black C. argus were mainly enriched in steroid biosynthesis, glycolysis/gluconeogenesis, and glutathione and propanoate metabolism pathways 24 h after infection. In contrast, differential genes in white C. argus were mainly enriched in pathways such as oxidative phosphorylation, pancreatic secretion, and protein digestion and absorption 24 h after infection. After A. hydrophila infection, white C. argus had higher mortality, more severe oxidative stress and inflammatory responses, and lower antioxidant capacity than black C. argus.

Abstract Image

两种不同颜色形态的乌鳢对急性嗜水气单胞菌感染的免疫反应
为了比较和分析乌鳢两种颜色形态之间免疫反应的差异,将鱼群分为四组:黑乌鳢+PBS(B-PBS)、黑乌鳢+嗜水气单胞菌(B-Ah)、白乌鳢+PBS(W-PBS)和白乌鳢+嗜水气单胞菌(W-Ah)。B-PBS 组和 W-PBS 组接受 100 μL PBS,而 B-Ah 组和 W-Ah 组在相同体积中接受 3.6 × 105 CFU/mL 的嗜水单胞菌。记录各组的死亡率,检测血浆生化指标的变化和肝脏免疫相关基因的表达,并使用转录组技术比较两种颜色的箭鱼在应激后的差异。B-PBS组和W-PBS组没有发生死亡。W-Ah组和B-Ah组的死亡率在注射嗜水蝇后呈先上升后下降的趋势。最高死亡率发生在 24 小时内,W-Ah 组高于 B-Ah 组。B-Ah 组和 W-Ah 组的 MDA 水平先上升后下降,而 SOD 和 T-AOC 则呈相反趋势。W-Ah 组和 W-PBS 组在 3、12 和 24 小时内的 MDA、6 至 96 小时内的 SOD 和 6 至 48 小时内的 T-AOC 含量有显著差异;B-PBS 组和 B-Ah 组在 12 小时内的血浆 MDA 和 T-AOC 含量以及 24 和 48 小时内的 SOD 含量有显著差异。在W-Ah组和B-Ah组中,肝脏中IL-1β和IL-8的表达水平呈现出先升高后降低的时间模式,在24小时后达到峰值水平,而IL-10则呈现出相反的模式。肝脏转录组分析表明,两种颜色的乌贼有显著差异。感染 24 小时后,黑箭鱼的差异基因主要富集在类固醇生物合成、糖酵解/葡萄糖生成以及谷胱甘肽和丙酸代谢途径中。相比之下,白乌贼的差异基因在感染 24 小时后主要富集在氧化磷酸化、胰腺分泌和蛋白质消化吸收等途径中。与黑银鱼相比,白银鱼感染蚜蝇藻后死亡率更高,氧化应激和炎症反应更严重,抗氧化能力更低。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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