Distribution and molecular analysis of Subtilase cytotoxin gene (subAB) variants in Shiga toxin-producing Escherichia coli (STEC) isolated from different sources in Iran.

IF 0.5 4区农林科学 Q4 VETERINARY SCIENCES

Veterinaria italiana Pub Date : 2024-07-31 DOI:10.12834/VetIt.3061.23280.2

Mahdi Askari Badouei, Maziar Jajarmi, Aria Narimani, Taghi Zahraei Salehi, Reza Ghanbarpour, Ali Nemati, Stefano Morabito, Alireza Koochakzadeh

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引用次数: 0

Abstract

Subtilase exhibits strong cytotoxicity that was first described in O113:H21 strain in Australia as a plasmid- encoded cytotoxin (subAB1). Subsequently, chromosomal variants including subAB2-1, subAB2-2, and subAB2-3 were described. We aimed to investigate the presence of subAB genes in a collection of Shiga toxin-producing Escherichia coli (STEC) strains (n=101) isolated from different sources in Iran. A collection of 101 archived STEC strains isolated from cattle (n=50), goats (n=25), sheep (n=15), wild captive animals (n=8: persian fallow deer, n=3; caspian pony, n=1; Macaca mulatta, n=4), and humans (n=3) during 2007-2016 were analyzed for the detection of different genes encoding the Subtilase variants, plasmidic and chromosomal virulence genes, phylogroups and serogroups. Overall, 57 isolates (56.4%) carried at least one variant of subAB. Most strains from small ruminants including 93% of sheep and 96% of caprine isolates carried at least one chromosomally encoded variant (subAB-2-1 and/or subAb2-2). In contrast, 12 cattle isolates (24%) only harbored the plasmid encoded variant (subAB1). STEC strains from other sources, including deer, pony and humans were positive for subAB-2-1 and/or subAb2-2. Our results reveal the presence of potentially pathogenic genotypes among locus of enterocyte effacement (LEE)-negative isolates, and some host specificity related to Subtilase variants and other virulence markers that may aid in source tracking of STEC during outbreak investigations.

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从伊朗不同来源分离的产志贺毒素大肠埃希菌（STEC）中胆碱酯酶细胞毒素基因（subAB）变体的分布和分子分析。

亚丝氨酸酶具有很强的细胞毒性，最早在澳大利亚的 O113:H21 菌株中被描述为一种质粒编码的细胞毒素（subAB1）。随后，包括 subAB2-1、subAB2-2 和 subAB2-3 在内的染色体变体也被描述出来。我们的目的是调查从伊朗不同来源分离的产志贺毒素大肠杆菌（STEC）菌株（n=101）中是否存在 subAB 基因。从牛（n=50）、山羊（n=25）、绵羊（n=15）、野生圈养动物（n=8：对 2007 年至 2016 年期间从牛（n=50）、山羊（n=25）、绵羊（n=15）、野生圈养动物（n=8：波斯秋鹿，n=3；里海矮种马，n=1；猕猴，n=4）和人类（n=3）中分离的 101 株 STEC 菌株进行了分析，以检测编码 Subtilase 变体的不同基因、质粒和染色体毒力基因、系统群和血清群。总体而言，57 个分离株（56.4%）至少携带一种 subAB 变体。大多数来自小型反刍动物的菌株，包括 93% 的绵羊和 96% 的山羊分离物，至少携带一种染色体编码变体（subAB-2-1 和/或 subAb2-2）。相比之下，12 个牛分离物（24%）只携带质粒编码变体（subAB1）。来自鹿、小马和人类等其他来源的 STEC 菌株对 subAB-2-1 和/或 subAb2-2 呈阳性。我们的研究结果表明，在肠细胞脱落位点（LEE）阴性的分离株中存在潜在的致病基因型，而且一些宿主特异性与 Subtilase 变体和其他毒力标记有关，这可能有助于在疫情调查期间追踪 STEC 的来源。

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来源期刊

Veterinaria italiana VETERINARY SCIENCES-

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The journal was created as the Croce Azzurra in 1950. A quarterly peer-reviewed journal devoted to veterinary public health and other aspects of veterinary science and medicine, Veterinaria Italiana is published by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell''Abruzzo e del Molise) in Teramo, Italy. The goal of the journal is to provide an international platform for veterinary public health information from Italy and other countries, particularly those in Eastern Europe and Africa, Asia and South America. Veterinarians and veterinary public health specialists are encouraged to share their knowledge and experience on this platform.