The chromatin accessibility landscape of mouse oocytes during configuration transition.

IF 5.9 1区生物学 Q2 CELL BIOLOGY

Cell Proliferation Pub Date : 2024-09-08 DOI:10.1111/cpr.13733

Shuai Zhu, Jiashuo Li, Xiuwan Wang, Yifei Jin, Hengjie Wang, Huiqing An, Hongzheng Sun, Longsen Han, Bin Shen, Qiang Wang

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Abstract

The transition of chromatin configuration in mammalian oocytes from a non-surrounded nucleolus (NSN) to a surrounded nucleolus (SN) is critical for acquiring the developmental competence. However, the genomic and epigenomic features underlying this process remain poorly understood. In the present study, we first establish the chromatin accessibility landscape of mouse oocytes from NSN to SN stage. Through the integrative analysis of multi-omics, we find that the establishment of DNA methylation in oocytes is independent of the dynamics of chromatin accessibility. In contrast, histone H3K4me3 status is closely associated with the dynamics of accessible regions during configuration transition. Furthermore, by focusing on the actively transcribed genes in NSN and SN oocytes, we discover that chromatin accessibility coupled with histone methylation (H3K4me3 and H3K27me3) participates in the transcriptional control during phase transition. In sum, our data provide a comprehensive resource for probing configuration transition in oocytes, and offer insights into the mechanisms determining chromatin dynamics and oocyte quality.

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小鼠卵母细胞配置转换过程中的染色质可及性图谱

哺乳动物卵母细胞中染色质构型从非环绕核仁（NSN）向环绕核仁（SN）的转变对于获得发育能力至关重要。然而，人们对这一过程的基因组和表观基因组特征仍然知之甚少。在本研究中，我们首先建立了小鼠卵母细胞从NSN到SN阶段的染色质可及性图谱。通过多组学的综合分析，我们发现卵母细胞中 DNA 甲基化的建立与染色质可及性的动态变化无关。相反，组蛋白 H3K4me3 的状态与构型转换过程中染色质可及区域的动态密切相关。此外，通过关注 NSN 和 SN 卵母细胞中活跃的转录基因，我们发现染色质可及性与组蛋白甲基化（H3K4me3 和 H3K27me3）共同参与了相变过程中的转录控制。总之，我们的数据为探究卵母细胞的构型转换提供了全面的资源，并为染色质动态和卵母细胞质量的决定机制提供了见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Proliferation 生物-细胞生物学

CiteScore

14.80

自引率

2.40%

发文量

198

审稿时长

1 months

期刊介绍： Cell Proliferation Focus: Devoted to studies into all aspects of cell proliferation and differentiation. Covers normal and abnormal states. Explores control systems and mechanisms at various levels: inter- and intracellular, molecular, and genetic. Investigates modification by and interactions with chemical and physical agents. Includes mathematical modeling and the development of new techniques. Publication Content: Original research papers Invited review articles Book reviews Letters commenting on previously published papers and/or topics of general interest By organizing the information in this manner, readers can quickly grasp the scope, focus, and publication content of Cell Proliferation.