Investigation of effect peripheral kisspeptin treatment on hypothalamo-pituitary-gonadal axis and hypothalamo-pituitary-adrenal axis in male rats.

IF 1.8 4区 生物学 Q3 BIOLOGY
Biologia futura Pub Date : 2024-12-01 Epub Date: 2024-09-09 DOI:10.1007/s42977-024-00241-3
Zafer Sahin, Osman Aktas, Omer Faruk Kalkan, Gokhan Cuce, Ahmet Alver, Elif Sahin, Seniz Erdem, Neslihan Saglam, Zulfikare Isik Solak Gormus, Selim Kutlu
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Abstract

Kisspeptin is an endogenous peptide hormone that is the most potent stimulator of the hypothalamo-pituitary-gonadal (HPG) axis. The HPG axis can be suppressed by the activation of the hypothalamo-pituitary-adrenal (HPA) axis. The physiological role of kisspeptin in the interaction of the HPG axis and the HPA axis is not fully understood yet. The purpose of the current study was to investigate the possible effects of peripheral injection (intraperitoneally) of kisspeptin on HPG axis and HPA axis activity as well. Adult male Wistar rats were randomly divided into seven groups as sham (control), kisspeptin (10 nmol), p234 (10 nmol), kisspeptin + p234, kisspeptin + antalarmin (10 mg/kg), kisspeptin + astressin2b (100 μg/kg), and kisspeptin + atosiban (0.250 mg/kg) (n = 10 each group). At the end of the experiment, the hypothalamus, pituitary gland, and serum samples of the rats were collected. Serum follicle-stimulating hormone and luteinizing hormone levels of the kisspeptin, kisspeptin + antalarmin and kisspeptin + astressin2b groups were significantly higher than the control group. Serum testosterone levels were significantly higher in the kisspeptin, kisspeptin + antalarmin, kisspeptin + astressin2b, and kisspeptin + atosiban groups that compared to the control group. There was no a significant difference in corticotropic releasing hormone immunoreactivity in the paraventricular nucleus of the hypothalamus, serum adrenocorticotropic hormone and corticosterone concentrations among all groups. Moreover, no significant difference was found in the concentration of pituitary oxytocin. Our results suggest that peripheral kisspeptin injection induces an activation in the HPG axis, but not in the HPA axis in male rats.

Abstract Image

研究外周kisspeptin处理对雄性大鼠下丘脑-垂体-性腺轴和下丘脑-垂体-肾上腺轴的影响
基斯肽是一种内源性肽类激素,是下丘脑-垂体-性腺轴(HPG)最有效的刺激物。下丘脑-垂体-肾上腺(HPA)轴的激活可抑制 HPG 轴。目前还不完全清楚Kisspeptin在HPG轴和HPA轴相互作用中的生理作用。本研究旨在探讨外周注射(腹腔注射)吻肽对 HPG 轴和 HPA 轴活动的可能影响。将成年雄性Wistar大鼠随机分为假(对照组)、kisspeptin(10 nmol)、p234(10 nmol)、kisspeptin + p234、kisspeptin + antalarmin(10 mg/kg)、kisspeptin + astressin2b(100 μg/kg)和kisspeptin + atosiban(0.250 mg/kg)七组(每组n = 10)。实验结束后,收集大鼠的下丘脑、垂体和血清样本。吻肽、吻肽+安他敏和吻肽+Astressin2b组的血清促卵泡激素和促黄体生成素水平显著高于对照组。与对照组相比,kisspeptin 组、kisspeptin + antalmin 组、kisspeptin + astressin2b 组和 kisspeptin + atosiban 组的血清睾酮水平明显更高。各组下丘脑室旁核的促肾上腺皮质激素释放激素免疫活性、血清促肾上腺皮质激素和皮质酮浓度均无明显差异。此外,垂体催产素的浓度没有明显差异。我们的研究结果表明,注射外周吻肽能诱导雄性大鼠的 HPG 轴活化,但不能诱导 HPA 轴活化。
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来源期刊
Biologia futura
Biologia futura Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
3.50
自引率
0.00%
发文量
27
期刊介绍: How can the scientific knowledge we possess now influence that future? That is, the FUTURE of Earth and life − of humankind. Can we make choices in the present to change our future? How can 21st century biological research ask proper scientific questions and find solid answers? Addressing these questions is the main goal of Biologia Futura (formerly Acta Biologica Hungarica). In keeping with the name, the new mission is to focus on areas of biology where major advances are to be expected, areas of biology with strong inter-disciplinary connection and to provide new avenues for future research in biology. Biologia Futura aims to publish articles from all fields of biology.
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