Complete genome sequence of grapevine yellow speckle viroid 3, a novel apscaviroid infecting grapevine, characterized by high-throughput sequencing

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2024-09-09 DOI:10.1007/s00705-024-06128-x

Malyaj R Prajapati, Pooja Thapa, Damini Diksha, Susheel Kumar Sharma, Nitika Gupta, Virendra Kumar Baranwal

引用次数: 0

Abstract

A novel grapevine viroid was discovered in an asymptomatic grapevine of Indian rootstocks. The whole genome sequence of the viroid (370 nt) was determined by high-throughput sequencing as well as RT-PCR followed by cloning and Sanger sequencing. The terminal conserved region (TCR), central conserved region (CCR) upper strand, and CCR lower strand are conserved regions found in the viroid that are unique to the members of the genus Apscaviroid. Based on our findings and the demarcation criteria for viroids, the novel viroid, which we have tentatively named "grapevine yellow speckle viroid 3" is a putative new member of the genus Apscaviroid.

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通过高通量测序鉴定葡萄黄斑病毒 3 的完整基因组序列，这是一种感染葡萄的新型 apscaviroid。

在一种无症状的印度砧木葡萄藤中发现了一种新型葡萄病毒。该病毒的全基因组序列（370 nt）是通过高通量测序、RT-PCR、克隆和桑格测序确定的。末端保守区（TCR）、中央保守区（CCR）上层链和中央保守区下层链是病毒体内发现的保守区，是 Apscaviroid 属成员所独有的。根据我们的研究结果和病毒病的划分标准，我们将这种新型病毒病暂时命名为 "葡萄黄斑病病毒 3 号"，它是 Apscaviroid 属的一个推定新成员。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.